Literature DB >> 833016

A comparison between the one-step and the two-step copper thiocholine procedure for the cytochemical localization of cholinesterases.

B Klinar, M Brzin.   

Abstract

A comparison between the one-step and the two step copper thiocholine procedure for the subcellular localization of cholinesterases was undertaken. The results indicate that only under experimental conditions with short incubation times and precise control of the conversion into sulphide is the localization of the primary precipitate and that of the secondary precipitate identical. It was concluded that the conversion of the primary precipitate into Cu-sulphide is not necessary and can lead to artefacts.

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Year:  1977        PMID： 833016     DOI： 10.1007/BF00507124

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  10 in total

1.  Cytochemical localization of cholinesterase activity at the giant synapse of the squid.

Authors:  M Brzin; V M Tennyson; W D Dettbarn
Journal:  Histochemistry       Date:  1975-06-09

2.  PREPARATION OF THICK SECTIONS FOR CYTOCHEMISTRY AND ELECTRON MICROSCOPY BY A NON-FREEZING TECHNIQUE.

Authors:  R E SMITH; M G FARQUHAR
Journal:  Nature       Date:  1963-11-16       Impact factor: 49.962

3.  A modification of the thiocholine method for the determination of cholinesterase. I. Biochemical evaluation of selective inhibitors.

Authors:  B HOLMSTEDT
Journal:  Acta Physiol Scand       Date:  1957-10-22

4.  [Histochemical studies on acetylcholine- and choline-esterases. III. Localization in the heart of mammals].

Authors:  E MOHR; M A GEREBTZOFF
Journal:  Acta Anat (Basel)       Date:  1954

5.  Attempts to demonstrate acetylcholinesterase activity in blood and bone-marrow cells by a modified thiocholine technique.

Authors:  J ZAJICEK; B SYLVEN; N DATTA
Journal:  J Histochem Cytochem       Date:  1954-03       Impact factor: 2.479

6.  A histochemical method for localizing cholinesterase activity.

Authors:  G B KOELLE; J A FRIEDENWALD
Journal:  Proc Soc Exp Biol Med       Date:  1949-04

7.  An electron-microscopic study of cholinesterase distribution in the rat adrenal medulla.

Authors:  P R Lewis; C C Shute
Journal:  J Microsc       Date:  1969       Impact factor: 1.758

8.  The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.

Authors:  E S REYNOLDS
Journal:  J Cell Biol       Date:  1963-04       Impact factor: 10.539

9.  ACETYLCHOLINESTERASE IN FROG SYMPATHETIC AND DORSAL ROOT GANGLIA : A Study by Electron Microscope Cytochemistry and Microgasometric Analysis with the Magnetic Diver.

Authors:  M Brzin; V M Tennyson; P E Duffy
Journal:  J Cell Biol       Date:  1966-11-01       Impact factor: 10.539

10.  The appearance of acetylcholinesterase in the myotome of the embryonic rabbit. An electron microscope cytochemical and biochemical study.

Authors:  V M Tennyson; M Brzin; P Slotwiner
Journal:  J Cell Biol       Date:  1971-12       Impact factor: 10.539
  10 in total

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