Literature DB >> 8329683

Physical mapping of a region in the soybean (Glycine max) genome containing duplicated sequences.

R P Funke1, A Kolchinsky, P M Gresshoff.   

Abstract

Pulsed-field gel electrophoresis (PFGE) was used to study a cluster of molecular markers in the soybean genome. There were 550 kb per centimorgan (cM) in the cluster, which is close to the calculated average for the whole genome. The analysis was complicated by the presence of duplicated sequences, and some ambiguities arising from this were resolved by using second-dimension conventional electrophoresis to relate physical maps to the RFLP map of soybean. The results show that there is a high degree of conservation of 'rare cutter' sites between homoeologous regions. Finally, PFGE can confirm physical linkage of monomorphic copies of markers, which can aid in the study and comparison of homoeologous regions that are invisible to RFLP analysis.

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Year:  1993        PMID： 8329683     DOI： 10.1007/bf00015974

Source DB:  PubMed          Journal:  Plant Mol Biol        ISSN： 0167-4412            Impact factor:   4.076


  16 in total

1.  Identification of the genomic locations of duplicate nucleotide sequences in maize by analysis of restriction fragment length polymorphisms.

Authors:  T Helentjaris; D Weber; S Wright
Journal:  Genetics       Date:  1988-02       Impact factor: 4.562

2.  The genetic locus controlling supernodulation in soybean (Glycine max L.) co-segregates tightly with a cloned molecular marker.

Authors:  D Landau-Ellis; S Angermüller; R Shoemaker; P M Gresshoff
Journal:  Mol Gen Genet       Date:  1991-08

3.  Strategies for mapping large regions of Mammalian genomes.

Authors:  M Burmeister
Journal:  Methods Mol Biol       Date:  1992

4.  Two-Dimensional DNA Electrophoresis (2D-DE) for Mammalian DNA.

Authors:  M A Walter; D W Cox
Journal:  Methods Mol Biol       Date:  1992

5.  Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

Authors:  D C Schwartz; C R Cantor
Journal:  Cell       Date:  1984-05       Impact factor: 41.582

6.  Restriction fragment length polymorphism diversity in soybean.

Authors:  P Keim; R C Shoemaker; R G Palmer
Journal:  Theor Appl Genet       Date:  1989-06       Impact factor: 5.699

7.  The genomic relationship between Glycine max (L.) Merr. and G. soja Sieb. and Zucc. as revealed by pachytene chromosome analysis.

Authors:  R J Singh; T Hymowitz
Journal:  Theor Appl Genet       Date:  1988-11       Impact factor: 5.699

8.  Correlation of genetic and physical structure in the region surrounding the I2 Fusarium oxysporum resistance locus in tomato.

Authors:  G Segal; M Sarfatti; M A Schaffer; N Ori; D Zamir; R Fluhr
Journal:  Mol Gen Genet       Date:  1992-01

9.  Inheritance and Organization of Glycinin Genes in Soybean.

Authors:  T. J. Cho; C. S. Davies; N. C. Nielsen
Journal:  Plant Cell       Date:  1989-03       Impact factor: 11.277

10.  Structure and chromosomal arrangement of leghemoglobin genes in kidney bean suggest divergence in soybean leghemoglobin gene loci following tetraploidization.

Authors:  J S Lee; D P Verma
Journal:  EMBO J       Date:  1984-12-01       Impact factor: 11.598
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  7 in total

1.  A major satellite DNA of soybean is a 92-base pairs tandem repeat.

Authors:  A Kolchinsky; P M Gresshoff
Journal:  Theor Appl Genet       Date:  1995-04       Impact factor: 5.699

2.  Variation in the ratio of physical to genetic distance in intervals adjacent to the Mla locus on barley chromosome 1H.

Authors:  R A DeScenzo; R P Wise
Journal:  Mol Gen Genet       Date:  1996-06-24

3.  Characterization and application of soybean YACs to molecular cytogenetics.

Authors:  T Zhu; I Shi; R P Funke; P M Gresshoff; P Keim
Journal:  Mol Gen Genet       Date:  1996-09-25

4.  Molecular marker technologies for plant improvement.

Authors:  P Winter; G Kahl
Journal:  World J Microbiol Biotechnol       Date:  1995-07       Impact factor: 3.312

5.  High EDTA concentrations cause entrapment of small DNA molecules in the compression zone of pulsed field gels, resulting in smaller than expected insert sizes in YACs prepared from size selected DNA.

Authors:  R P Funke; A Kolchinsky; P M Gresshoff
Journal:  Nucleic Acids Res       Date:  1994-07-11       Impact factor: 16.971

6.  Hypomethylated sequences: characterization of the duplicate soybean genome.

Authors:  T Zhu; J M Schupp; A Oliphant; P Keim
Journal:  Mol Gen Genet       Date:  1994-09-28

7.  Organization of soybean chalcone synthase gene clusters and characterization of a new member of the family.

Authors:  S Akada; S K Dube
Journal:  Plant Mol Biol       Date:  1995-10       Impact factor: 4.076
  7 in total

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