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Literature DB >> 8329682

Molecular cloning and analysis of a cDNA coding for the bifunctional dihydrofolate reductase-thymidylate synthase of Daucus carota.

M Luo¹, P Piffanelli, L Rastelli, R Cella.

Abstract

Molecular cloning of dihydrofolate reductase-thymidylate synthase (DHFR-TS) of Daucus carota was achieved by immunoscreening of a cDNA library obtaining a 2 kbp clone which contains an open reading frame of 1528 bp. Comparison of the deduced amino acid sequence with those from other sources revealed the presence of motifs typical of DHFR and TS thus confirming the bifunctional nature of the carrot protein. As in other organisms, a higher degree of conservation was observed in the TS domain. Analysis of the dhfr-ts gene content in carrot revealed the presence of several copies per diploid genome.

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Year: 1993 PMID： 8329682 DOI： 10.1007/bf00015973

Source DB: PubMed Journal: Plant Mol Biol ISSN： 0167-4412 Impact factor: 4.076

31 in total

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Journal: Proc Natl Acad Sci U S A Date: 1987-12 Impact factor: 11.205

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Authors: G F Maley; F Maley; C M Baugh
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Journal: J Biol Chem Date: 1987-04-15 Impact factor: 5.157

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Authors: B I Schweitzer; A P Dicker; J R Bertino
Journal: FASEB J Date: 1990-05 Impact factor: 5.191

5. Photoaffinity analogues of methotrexate as folate antagonist binding probes. 1. Photoaffinity labeling of murine L1210 dihydrofolate reductase and amino acid sequence of the binding region.

Authors: E M Price; P L Smith; T E Klein; J H Freisheim
Journal: Biochemistry Date: 1987-07-28 Impact factor: 3.162

6. Proteolytic and partial sequencing studies of the bifunctional dihydrofolate reductase-thymidylate synthase from Daucus carota.

Authors: R Cella; D Carbonera; R Orsi; G Ferri; P Iadarola
Journal: Plant Mol Biol Date: 1991-06 Impact factor: 4.076

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Authors: M E Fling; J Kopf; C A Richards
Journal: Gene Date: 1988-03-31 Impact factor: 3.688

8. Primary structure of the Escherichia coli thyA gene and its thymidylate synthase product.

Authors: M Belfort; G Maley; J Pedersen-Lane; F Maley
Journal: Proc Natl Acad Sci U S A Date: 1983-08 Impact factor: 11.205

9. Purification, characterization, cloning, and amino acid sequence of the bifunctional enzyme 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase from Escherichia coli.

Authors: L D'Ari; J C Rabinowitz
Journal: J Biol Chem Date: 1991-12-15 Impact factor: 5.157

10. Daucus carota cells contain a dihydrofolate reductase: thymidylate synthase bifunctional polypeptide.

Authors: R Cella; E Nielsen; B Parisi
Journal: Plant Mol Biol Date: 1988-07 Impact factor: 4.076

11 in total

1. Cloning and characterisation of a carrot cDNA coding for a WD repeat protein homologous to Drosophila fizzy, human p55CDC and yeast CDC20 proteins.

Authors: M Luo; S Costa; G Bernacchia; R Cella
Journal: Plant Mol Biol Date: 1997-05 Impact factor: 4.076

2. Multiple transcription start sites of the carrot dihydrofolate reductase-thymidylate synthase gene, and sub-cellular localization of the bifunctional protein.

Authors: M Luo; R Orsi; E Patrucco; S Pancaldi; R Cella
Journal: Plant Mol Biol Date: 1997-03 Impact factor: 4.076

3. Mapping and expression of a bifunctional thymidylate synthase, dihydrofolate reductase gene from maize.

Authors: K Cox; D Robertson; R Fites
Journal: Plant Mol Biol Date: 1999-12 Impact factor: 4.076

4. Effects of sulfanilamide and methotrexate on 13C fluxes through the glycine decarboxylase/serine hydroxymethyltransferase enzyme system in arabidopsis.

Authors: V Prabhu; K B Chatson; H Lui; G D Abrams; J King
Journal: Plant Physiol Date: 1998-01 Impact factor: 8.340

5. Towards establishment of a plant-based model to assess the novel anti-cancerous lead molecule(s): An in silico, in vivo and in vitro assessment of some potential anti-cancerous drugs on Lathyrus sativus L.

Authors: Aveek Samanta; Saptadipa Banerjee; Tilak Raj Maity; Jangala Jahnavi; Siraj Datta
Journal: Protoplasma Date: 2022-02-23 Impact factor: 3.186

6. Identification of a thymidylate synthase gene within the genome of Chilo iridescent virus.

Authors: K Müller; C A Tidona; U Bahr; G Darai
Journal: Virus Genes Date: 1998 Impact factor: 2.332

7. Cloning and molecular analysis of the bifunctional dihydrofolate reductase-thymidylate synthase gene in the ciliated protozoan Paramecium tetraurelia.

Authors: I M Schlichtherle; D S Roos; J L Van Houten
Journal: Mol Gen Genet Date: 1996-04-10