Literature DB >> 8329220

Selective isolation of highly polymorphic (dC-dA)n.(dG-dT)n microsatellites by stringent hybridization.

M Iizuka1, R Makino, T Sekiya, K Hayashi.   

Abstract

We defined a hybridization condition for isolation of clones carrying long (dC-dA)n.(dG-dT)n microsatellites from a genomic plasmid library. By using (dC-dA)20 oligonucleotide as a probe and hybridizing under stringent conditions, more than two-thirds of the obtained plasmid carried the repeats that were longer than (dC-dA)14 and highly polymorphic.

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Year:  1993        PMID： 8329220     DOI： 10.1016/1050-3862(93)90017-d

Source DB:  PubMed          Journal:  Genet Anal Tech Appl        ISSN： 1050-3862


  3 in total

1.  Computerized polymorphic marker identification: experimental validation and a predicted human polymorphism catalog.

Authors:  J W Fondon; G M Mele; R I Brezinschek; D Cummings; A Pande; J Wren; K M O'Brien; K C Kupfer; M H Wei; M Lerman; J D Minna; H R Garner
Journal:  Proc Natl Acad Sci U S A       Date:  1998-06-23       Impact factor: 11.205

2.  Linkage-disequilibrium mapping narrows the Fukuyama-type congenital muscular dystrophy (FCMD) candidate region to <100 kb.

Authors:  T Toda; M Miyake; K Kobayashi; K Mizuno; K Saito; M Osawa; Y Nakamura; I Kanazawa; Y Nakagome; K Tokunaga; Y Nakahori
Journal:  Am J Hum Genet       Date:  1996-12       Impact factor: 11.025

3.  A genomic clone containing a telomere array maps near the centromere of mouse chromosome 6.

Authors:  C H Yen; Y Matsuda; V M Chapman; R W Elliott
Journal:  Mamm Genome       Date:  1995-02       Impact factor: 2.957
  3 in total

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