Literature DB >> 8329181

Purification and partial characterization of a rat kidney aldehyde dehydrogenase that oxidizes retinal to retinoic acid.

J Labrecque1, P V Bhat, A Lacroix.   

Abstract

A NAD-dependent aldehyde dehydrogenase (EC 1.2.1.3) which catalyzes the oxidation of retinal to retinoic acid was purified to homogeneity from rat kidney by using Affi-Gel blue affinity chromatography and chromatofocusing, followed by Mono-Q anion-exchange chromatography. The apparent molecular weight of the native enzyme determined by size-exclusion fast protein liquid chromatography was 140,000. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis gave a subunit molecular weight of 53,000. The isoelectric point as measured by chromatofocusing was 8.5. The enzyme also catalyzed the oxidation of acetaldehyde, but showed much lower Km value for the retinal substrate. We suggest that aldehyde dehydrogenase found in the kidney may be a specific retinal dehydrogenase, involved in vitamin A metabolism.

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Year:  1993        PMID： 8329181     DOI： 10.1139/o93-013

Source DB:  PubMed          Journal:  Biochem Cell Biol        ISSN： 0829-8211            Impact factor:   3.626


  10 in total

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