Literature DB >> 8320242

Simple phagemid-based system for generating allele replacements in Escherichia coli.

S Slater1, R Maurer.   

Abstract

We describe a phagemid-based system for rapidly generating chromosomal allele replacements in two steps. The system utilizes simple vector-determined selection procedures for each step and requires only that the target strain be male and sup+ (nonsuppressing for amber mutations).

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Year:  1993        PMID： 8320242      PMCID： PMC204859          DOI： 10.1128/jb.175.13.4260-4262.1993

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  6 in total

1.  Allelic exchange in Escherichia coli using the Bacillus subtilis sacB gene and a temperature-sensitive pSC101 replicon.

Authors:  I C Blomfield; V Vaughn; R F Rest; B I Eisenstein
Journal:  Mol Microbiol       Date:  1991-06       Impact factor: 3.501

2.  Cloning vectors that yield high levels of single-stranded DNA for rapid DNA sequencing.

Authors:  R J Zagursky; M L Berman
Journal:  Gene       Date:  1984-02       Impact factor: 3.688

3.  Replication of a plasmid containing two origins of bacteriophage.

Authors:  G P Dotto; K Horiuchi
Journal:  J Mol Biol       Date:  1981-11-25       Impact factor: 5.469

4.  An nptI-sacB-sacR cartridge for constructing directed, unmarked mutations in gram-negative bacteria by marker exchange-eviction mutagenesis.

Authors:  J L Ried; A Collmer
Journal:  Gene       Date:  1987       Impact factor: 3.688

5.  Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

Authors:  C Yanisch-Perron; J Vieira; J Messing
Journal:  Gene       Date:  1985       Impact factor: 3.688

6.  The functional origin of bacteriophage f1 DNA replication. Its signals and domains.

Authors:  G P Dotto; K Horiuchi; N D Zinder
Journal:  J Mol Biol       Date:  1984-02-05       Impact factor: 5.469
  6 in total
  33 in total

1.  Construction and initial characterization of Escherichia coli strains with few or no intact chromosomal rRNA operons.

Authors:  T Asai; C Condon; J Voulgaris; D Zaporojets; B Shen; M Al-Omar; C Squires; C L Squires
Journal:  J Bacteriol       Date:  1999-06       Impact factor: 3.490

2.  Identification and characterization of a new lipoprotein, NlpI, in Escherichia coli K-12.

Authors:  M Ohara; H C Wu; K Sankaran; P D Rick
Journal:  J Bacteriol       Date:  1999-07       Impact factor: 3.490

3.  Signal transduction pathways in response to protein misfolding in the extracytoplasmic compartments of E. coli: role of two new phosphoprotein phosphatases PrpA and PrpB.

Authors:  D Missiakas; S Raina
Journal:  EMBO J       Date:  1997-04-01       Impact factor: 11.598

4.  Stable co-existence of separate replicons in Escherichia coli is dependent on once-per-cell-cycle initiation.

Authors:  Kirsten Skarstad; Anders Løbner-Olesen
Journal:  EMBO J       Date:  2003-01-02       Impact factor: 11.598

5.  Convenient and reversible site-specific targeting of exogenous DNA into a bacterial chromosome by use of the FLP recombinase: the FLIRT system.

Authors:  L C Huang; E A Wood; M M Cox
Journal:  J Bacteriol       Date:  1997-10       Impact factor: 3.490

6.  Multiple effects of S13 in modulating the strength of intersubunit interactions in the ribosome during translation.

Authors:  Anthony R Cukras; Rachel Green
Journal:  J Mol Biol       Date:  2005-04-12       Impact factor: 5.469

7.  The Escherichia coli baby cell column: a novel cell synchronization method provides new insight into the bacterial cell cycle.

Authors:  David Bates; Jessica Epstein; Erik Boye; Karen Fahrner; Howard Berg; Nancy Kleckner
Journal:  Mol Microbiol       Date:  2005-07       Impact factor: 3.501

8.  DNA sequence context affects UV-induced mutagenesis in Escherichia coli.

Authors:  Wai Bing Mak; Douglas Fix
Journal:  Mutat Res       Date:  2007-10-13       Impact factor: 2.433

9.  Examination of the Tn5 transposase overproduction phenotype in Escherichia coli and localization of a suppressor of transposase overproduction killing that is an allele of rpoH.

Authors:  H Yigit; W S Reznikoff
Journal:  J Bacteriol       Date:  1997-03       Impact factor: 3.490

10.  holE, the gene coding for the theta subunit of DNA polymerase III of Escherichia coli: characterization of a holE mutant and comparison with a dnaQ (epsilon-subunit) mutant.

Authors:  S C Slater; M R Lifsics; M O'Donnell; R Maurer
Journal:  J Bacteriol       Date:  1994-02       Impact factor: 3.490
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