A M Delany1, C E Brinckerhoff. 1. Department of Medicine, Dartmouth Medical School, Hanover, New Hampshire 03755-3844.
Abstract
OBJECTIVE: We documented the effect of the retinoid (4-hydroxyphenyl)retinamide on collagen expression in a tissue culture and in an animal model of scleroderma. METHODS: We used RNA analysis, chloramphenicol acetyltransferase assays, organ culture, and histologic evaluation. RESULTS: We showed that (4-hydroxyphenyl)retinamide decreases alpha 1(I) collagen messenger RNA and transcription in cultured cells, and decreases collagen levels in the dermis of tight-skin mice. CONCLUSION: These results provide a basis for further experiments to address the efficacy of (4-hydroxyphenyl)retinamide in the treatment of scleroderma.
OBJECTIVE: We documented the effect of the retinoid (4-hydroxyphenyl)retinamide on collagen expression in a tissue culture and in an animal model of scleroderma. METHODS: We used RNA analysis, chloramphenicol acetyltransferase assays, organ culture, and histologic evaluation. RESULTS: We showed that (4-hydroxyphenyl)retinamide decreases alpha 1(I) collagen messenger RNA and transcription in cultured cells, and decreases collagen levels in the dermis of tight-skin mice. CONCLUSION: These results provide a basis for further experiments to address the efficacy of (4-hydroxyphenyl)retinamide in the treatment of scleroderma.