| Literature DB >> 8313476 |
M Wassenegger1, S Heimes, L Riedel, H L Sänger.
Abstract
One monomeric and three oligomeric potato spindle tuber viroid (PSTVd) cDNA units were introduced into the tobacco genome via the Agrobacterium-mediated leaf-disc transformation. Southern analysis of the integrates revealed that only their PSTVd-specific sequences become fully methylated, whereas the flanking T-DNA and the genomic plant DNA remain unaltered. Viroid cDNA methylation could only be observed after autonomous viroid RNA-RNA replication had taken place in these plants. These findings demonstrate that a mechanism of de novo methylation of genes might exist that can be induced and targeted in a sequence-specific manner by their own mRNA.Entities:
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Year: 1994 PMID： 8313476 DOI： 10.1016/0092-8674(94)90119-8
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582