D B Seifer1, R Romero, D Berlinsky, R V Haning. 1. Division of Reproductive Endocrinology, Women and Infants Hospital, Brown University School of Medicine, Providence, Rhode Island.
Abstract
PROBLEM: The purpose of this study was to determine if it was possible to use commercially available enzyme-linked immunosorbent assay (ELISA) kits to quantitate specific cytokine production from individual embryos. METHODS: We examined the supernatants of individual two to six cell preimplantation human embryos using commercially available kits for IL-1 alpha, IL-1 beta, IL-6, and LIF. RESULTS: Cytokines were undetectable in all media samples analyzed. CONCLUSION: While it is possible that individual preimplantation embryos may produce cytokines, we believe these concentrations are below the detectable limits of the commercial assays used in this study.
PROBLEM: The purpose of this study was to determine if it was possible to use commercially available enzyme-linked immunosorbent assay (ELISA) kits to quantitate specific cytokine production from individual embryos. METHODS: We examined the supernatants of individual two to six cell preimplantation human embryos using commercially available kits for IL-1 alpha, IL-1 beta, IL-6, and LIF. RESULTS: Cytokines were undetectable in all media samples analyzed. CONCLUSION: While it is possible that individual preimplantation embryos may produce cytokines, we believe these concentrations are below the detectable limits of the commercial assays used in this study.
Authors: G Chaouat; E Menu; B Mognetti; M Moussa; V Cayol; Y Mostefaoui; S Dubanchet; P Khadel; J L Voluménie; C B Rongiéres; G L Delage Journal: Infect Dis Obstet Gynecol Date: 1997