Literature DB >> 8310820

A simple method of rapid freezing adequately preserves brain tissue for immunocytochemistry, light and electron microscopic examination.

D Nochlin1, A P Mackenzie, E M Bryant, T H Norwood, S M Sumi.   

Abstract

A simple and reproducible method for cryopreservation of brain tissue from patients with Alzheimer's disease is described. Fresh brain slices (1 cm thick) obtained less than 6 h postmortem are placed in sealed plastic bags, sandwiched between 0.3-cm-thick aluminium sheets, and frozen by placing the entire "sandwich" between layers of dry ice pellets. The frozen brain slices are stored at -85 degrees C. Specific anatomic areas can be retrieved at any time for light and electron microscopic, immunocytochemical, autoradiographic and neurochemical studies.

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Year:  1993        PMID： 8310820     DOI： 10.1007/bf00294305

Source DB:  PubMed          Journal:  Acta Neuropathol        ISSN： 0001-6322            Impact factor:   17.088


  11 in total

1.  ELECTRON MICROSCOPY AFTER RAPID FREEZING ON A METAL SURFACE AND SUBSTITUTION FIXATION.

Authors:  A VANHARREVELD; J CROWELL
Journal:  Anat Rec       Date:  1964-07

2.  EFFECTS OF FREEZING AND THAWING ON THE STRUCTURE, CHEMICAL CONSTITUTION, AND FUNCTION OF CYTOPLASMIC STRUCTURES.

Authors:  B F TRUMP; D E YOUNG; E A ARNOLD; R E STOWELL
Journal:  Fed Proc       Date:  1965 Mar-Apr

3.  Formic acid pretreatment enhances immunostaining of cerebral and systemic amyloids.

Authors:  T Kitamoto; K Ogomori; J Tateishi; S B Prusiner
Journal:  Lab Invest       Date:  1987-08       Impact factor: 5.662

4.  Diffuse type of senile plaques in the brains of Alzheimer-type dementia.

Authors:  H Yamaguchi; S Hirai; M Morimatsu; M Shoji; Y Harigaya
Journal:  Acta Neuropathol       Date:  1988       Impact factor: 17.088

5.  Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.

Authors:  S M Hsu; L Raine; H Fanger
Journal:  J Histochem Cytochem       Date:  1981-04       Impact factor: 2.479

6.  Vacuolar change in Alzheimer's disease.

Authors:  T W Smith; U Anwer; U DeGirolami; D A Drachman
Journal:  Arch Neurol       Date:  1987-12

7.  Ultrastructural observations on tissues processed by a quick-freezing, rapid-drying method: comparison with conventional specimen preparation.

Authors:  L Terracio; P W Bankston; J A McAteer
Journal:  Cryobiology       Date:  1981-02       Impact factor: 2.487

8.  Electron microscopic and immunocytochemical study of rapidly frozen, freeze-substituted neural lobes of rats.

Authors:  M Tian; J F Reger; W E Armstrong
Journal:  J Neurocytol       Date:  1991-02

9.  Creutzfeldt-Jakob disease virus isolations from the Gerstmann-Sträussler syndrome with an analysis of the various forms of amyloid plaque deposition in the virus-induced spongiform encephalopathies.

Authors:  C L Masters; D C Gajdusek; C J Gibbs
Journal:  Brain       Date:  1981-09       Impact factor: 13.501

10.  A STUDY OF EXTRACELLULAR SPACE IN CENTRAL NERVOUS TISSUE BY FREEZE-SUBSTITUTION.

Authors:  A VANHARREVELD; J CROWELL; S K MALHOTRA
Journal:  J Cell Biol       Date:  1965-04       Impact factor: 10.539
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  1 in total

1.  Clinical and neuropathological features of the arctic APP gene mutation causing early-onset Alzheimer disease.

Authors:  Hans Basun; Nenad Bogdanovic; Martin Ingelsson; Ove Almkvist; Jan Näslund; Karin Axelman; Thomas D Bird; David Nochlin; Gerard D Schellenberg; Lars-Olof Wahlund; Lars Lannfelt
Journal:  Arch Neurol       Date:  2008-04
  1 in total

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