Simian agent 8 (SA8): morphogenesis and ultrastructure.

Electron microscopic studies on the morphogenesis of SA8 in primary rabbit brain cell cultures revealed that in early stages of infection, envelopment of nucleocapsids commonly occurred at the inner nuclear membrane. From the perinuclear space, enveloped virus particles moved into the cisternae of the endoplasmic reticulum (ER) in which they were transported, through the cytoplasm, to the plasma membrane. Alternatively, de-envelopment at the outer nuclear membrane and egress of naked capsids into the cytoplasm were frequently observed. Non-enveloped cytoplasmic capsids were also a consistent feature of cells in late stages of infection, when nuclear membranes became ruptured. In these cases, the envelopment of naked capsids took place by budding either into the cisternae of ER or into cytoplasmic vesicles and vacuoles, in which transport to and exocytosis at the cell membrane occurred. Budding at the cell membrane was rarely found. Capsids of enveloped particles were asymmetrically surrounded by an electron-dense layer which may be identical to the tegument. Because only enveloped cytoplasmic and free virions were tegumented we suggested that the tegumentation must occur during the envelopment (budding) into cytoplasmic vesicles and at the plasma membrane.