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Literature DB >> 8302941

Restriction map of a virulence-associated plasmid of Rhodococcus equi.

T Kanno¹, T Asawa, H Ito, S Takai, S Tsubaki, T Sekizaki.

Abstract

The restriction cleavage map of a virulence-associated plasmid pREAT701 of Rhodococcus equi was constructed with EcoRI and HindIII by means of cloning the restriction fragments, cross Southern hybridization with each fragment, and hybridization with probes generated by modified inverse PCR. The genetic region responsible for expression of virulence-associated 15- to 17-kilodalton antigens was determined.

Entities: Species

Mesh：

Substances：
DNA Primers

Year: 1993 PMID： 8302941 DOI： 10.1006/plas.1993.1065

Source DB: PubMed Journal: Plasmid ISSN： 0147-619X Impact factor: 3.466

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Cited

7 in total

1. Restriction enzyme analysis of the virulence plasmids of VapA-positive Rhodococcus equi strains isolated from humans and horses.

Authors: V M Nicholson; J F Prescott
Journal: J Clin Microbiol Date: 1997-03 Impact factor: 5.948

2. Epidemiology of Rhodococcus equi strains on Thoroughbred horse farms.

Authors: A C Morton; A P Begg; G A Anderson; S Takai; C Lämmler; G F Browning
Journal: Appl Environ Microbiol Date: 2001-05 Impact factor: 4.792

3. Molecular characterization of a lipid-modified virulence-associated protein of Rhodococcus equi and its potential in protective immunity.

Authors: C Tan; J F Prescott; M C Patterson; V M Nicholson
Journal: Can J Vet Res Date: 1995-01 Impact factor: 1.310

Restriction map of a virulence-associated plasmid of Rhodococcus equi.

1. Restriction enzyme analysis of the virulence plasmids of VapA-positive Rhodococcus equi strains isolated from humans and horses.

2. Epidemiology of Rhodococcus equi strains on Thoroughbred horse farms.

3. Molecular characterization of a lipid-modified virulence-associated protein of Rhodococcus equi and its potential in protective immunity.

4. Identification of intermediately virulent Rhodococcus equi isolates from pigs.

5. Attempts to find phenotypic markers of the virulence plasmid of Rhodococcus equi.

6. A physical map of the 85 kb virulence plasmid of Rhodococcus equi 103.

7. Identification of virulent Rhodococcus equi by amplification of gene coding for 15- to 17-kilodalton antigens.