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Literature DB >> 8302865

Evidence for a non-alpha-helical DNA-binding motif in the Rel homology region.

J Liu¹, M Sodeoka, W S Lane, G L Verdine.

Abstract

The Rel family of transcription factors serve as terminal messengers in a variety of developmental and receptor-mediated signaling pathways. These proteins are related by a domain of approximately 280 amino acids, the Rel homology region, which mediates dimerization and sequence-specific binding to DNA. Here we report the use of photocrosslinking and site-directed mutagenesis to identify specific contact partners in a Rel protein-DNA interface. Within the Rel homology region of NF-kappa B p50 (also known as KBF1), two amino acid residues were identified by photocrosslinking to adjacent bases in a beta-interferon regulatory element. Secondary structure analysis suggests that the DNA-binding motif of the Rel homology region comprises a beta-turn-beta structure, in contrast to the alpha-helical motifs so commonly observed in transcription factors.

Entities: Gene

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Year: 1994 PMID： 8302865 PMCID： PMC521422 DOI： 10.1073/pnas.91.3.908

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

43 in total

1. Cloning of a mitogen-inducible gene encoding a kappa B DNA-binding protein with homology to the rel oncogene and to cell-cycle motifs.

Authors: V Bours; J Villalobos; P R Burd; K Kelly; U Siebenlist
Journal: Nature Date: 1990-11-01 Impact factor: 49.962

2. Cloning of the p50 DNA binding subunit of NF-kappa B: homology to rel and dorsal.

Authors: S Ghosh; A M Gifford; L R Riviere; P Tempst; G P Nolan; D Baltimore
Journal: Cell Date: 1990-09-07 Impact factor: 41.582

3. The DNA binding subunit of NF-kappa B is identical to factor KBF1 and homologous to the rel oncogene product.

Authors: M Kieran; V Blank; F Logeat; J Vandekerckhove; F Lottspeich; O Le Bail; M B Urban; P Kourilsky; P A Baeuerle; A Israël
Journal: Cell Date: 1990-09-07 Impact factor: 41.582

4. Structure of a mammalian c-rel protein deduced from the nucleotide sequence of murine cDNA clones.

Authors: R J Grumont; S Gerondakis
Journal: Oncogene Res Date: 1989

5. Refinement of Eco RI endonuclease crystal structure: a revised protein chain tracing.

Authors: Y C Kim; J C Grable; R Love; P J Greene; J M Rosenberg
Journal: Science Date: 1990-09-14 Impact factor: 47.728

6. Lac repressor-operator interaction. I. Equilibrium studies.

Authors: A D Riggs; H Suzuki; S Bourgeois
Journal: J Mol Biol Date: 1970-02-28 Impact factor: 5.469

7. The v-rel oncogene encodes a kappa B enhancer binding protein that inhibits NF-kappa B function.

Authors: D W Ballard; W H Walker; S Doerre; P Sista; J A Molitor; E P Dixon; N J Peffer; M Hannink; W C Greene
Journal: Cell Date: 1990-11-16 Impact factor: 41.582

8. Co-crystal structure of TBP recognizing the minor groove of a TATA element.

Authors: J L Kim; D B Nikolov; S K Burley
Journal: Nature Date: 1993-10-07 Impact factor: 49.962

9. Dorsal, an embryonic polarity gene in Drosophila, is homologous to the vertebrate proto-oncogene, c-rel.

Authors: R Steward
Journal: Science Date: 1987-10-30 Impact factor: 47.728

Review 10. NF-kappa B, KBF1, dorsal, and related matters.

Authors: T D Gilmore
Journal: Cell Date: 1990-09-07 Impact factor: 41.582

12 in total

1. Mutations in the v-Rel transactivation domain indicate altered phosphorylation and identify a subset of NF-kappaB-regulated cell death inhibitors important for v-Rel transforming activity.

Authors: Béatrice Rayet; Yongjun Fan; Céline Gélinas
Journal: Mol Cell Biol Date: 2003-03 Impact factor: 4.272

2. Molecular cloning of a small DNA binding protein with specificity for a tissue-specific negative element within the rps1 promoter.

Authors: D X Zhou; C Bisanz-Seyer; R Mache
Journal: Nucleic Acids Res Date: 1995-04-11 Impact factor: 16.971

3. NFATx, a novel member of the nuclear factor of activated T cells family that is expressed predominantly in the thymus.

Authors: E S Masuda; Y Naito; H Tokumitsu; D Campbell; F Saito; C Hannum; K Arai; N Arai
Journal: Mol Cell Biol Date: 1995-05 Impact factor: 4.272

4. An RNA-protein contact determined by 5-bromouridine substitution, photocrosslinking and sequencing.

Authors: M C Willis; K A LeCuyer; K M Meisenheimer; O C Uhlenbeck; T H Koch
Journal: Nucleic Acids Res Date: 1994-11-25 Impact factor: 16.971

5. The PEST-like sequence of I kappa B alpha is responsible for inhibition of DNA binding but not for cytoplasmic retention of c-Rel or RelA homodimers.

Authors: M K Ernst; L L Dunn; N R Rice
Journal: Mol Cell Biol Date: 1995-02 Impact factor: 4.272

6. Inhibition of NF-kappaB DNA binding by nitric oxide.

Authors: J R Matthews; C H Botting; M Panico; H R Morris; R T Hay
Journal: Nucleic Acids Res Date: 1996-06-15 Impact factor: 16.971

7. Crosslinking of double-stranded oligonucleotides containing O-methyl-substituted pyrophosphate groups to the HNF1 transcription factor in nuclear cell extract.

Authors: S A Kuznetsova; C Clusel; E Ugarte; I Elias; M Vasseur; M Blumenfeld; Z A Shabarova
Journal: Nucleic Acids Res Date: 1996-12-01 Impact factor: 16.971

8. A residue of the ETS domain mutated in the v-ets oncogene is essential for the DNA-binding and transactivating properties of the ETS-1 and ETS-2 proteins.

Authors: N Soudant; O Albagli; P Dhordain; A Flourens; D Stéhelin; D Leprince
Journal: Nucleic Acids Res Date: 1994-09-25 Impact factor: 16.971

9. Characterization of elements determining the dimerization properties of RelB and p50.

Authors: R P Ryseck; J Novotny; R Bravo
Journal: Mol Cell Biol Date: 1995-06 Impact factor: 4.272

10. Conformational changes induced by DNA binding of NF-kappa B.

Authors: J R Matthews; J Nicholson; E Jaffray; S M Kelly; N C Price; R T Hay
Journal: Nucleic Acids Res Date: 1995-09-11 Impact factor: 16.971