The candelabrum cell: a new interneuron in the cerebellar cortex.

A new cell type is described in silver-impregnated sections of the rat cerebellar cortex, uniformly distributed through all the cerebellar folia. The soma is rather small, roughly pyriform, vertically oriented, and squeezed, in a sandwich-like manner, between the Purkinje cell somata. One or two thick dendrites arise from the upper pole of the cell body and course through the entire molecular layer, dividing into a few, slightly oblique, branches that can reach the pia mater. These dendrites are covered with irregularly distributed spines. Some more slender dendrites emerge from the lower part of the cell body, or from the proximal trunk of a molecular dendrite, and spread tortuously for a short distance in the upper granular layer. A thick initial segment emerges directly from the soma or from the proximal portion of a dendrite, the axon winding then horizontally through or just above the Purkinje cell layer. During this horizontal course it gives off vertically oriented beaded branches ascending through the major part of the molecular layer. These branches, rather closely spaced, occupy different parasagittal planes, separated by about 10 to 30 microns. This axonal arborisation can thus be compared with a candelabrum. The peculiar three-dimensional spread of the axonal collaterals suggests a functional relationship between these branches and the dendritic trunks of neighbouring Purkinje cells. A comparative analysis of the morphological differences between this candelabrum interneuron and the other corticocerebellar interneurons found in the vicinity of the ganglionic layer confirms the specificity of this new cell class.