UNLABELLED: Iodine-123-iomazenil binding to benzodiazepine receptors in human brain was measured with SPECT using kinetic and equilibrium methods. METHODS: In the kinetic experiments (n = 6), regional time-activity curves after a single bolus injection of the tracer were fit to a three-compartment model to provide estimates of the rate constants K1 to k4. The binding potential (equal to the product of the receptor density and affinity) was derived from the rate constants. In the equilibrium method (n = 8), the tracer bolus injection was followed by a constant tracer infusion to induce a sustained equilibrium state. The regional equilibrium volume of distribution was calculated as the ratio of the regional brain concentration-to-the free parent tracer steady-state plasma concentration. In three experiments, a receptor-saturating dose of flumazenil was injected for direct measurement of the nondisplaceable compartment distribution volume. RESULTS: The kinetic and equilibrium method results were in good agreement in all regions investigated. Iodine-125-iomazenil binding potential measured in vitro in 12 postmortem samples was found to be consistent with SPECT in vivo measurements. CONCLUSION: These studies demonstrated the feasibility of quantification of receptor binding with SPECT.
UNLABELLED: Iodine-123-iomazenil binding to benzodiazepine receptors in human brain was measured with SPECT using kinetic and equilibrium methods. METHODS: In the kinetic experiments (n = 6), regional time-activity curves after a single bolus injection of the tracer were fit to a three-compartment model to provide estimates of the rate constants K1 to k4. The binding potential (equal to the product of the receptor density and affinity) was derived from the rate constants. In the equilibrium method (n = 8), the tracer bolus injection was followed by a constant tracer infusion to induce a sustained equilibrium state. The regional equilibrium volume of distribution was calculated as the ratio of the regional brain concentration-to-the free parent tracer steady-state plasma concentration. In three experiments, a receptor-saturating dose of flumazenil was injected for direct measurement of the nondisplaceable compartment distribution volume. RESULTS: The kinetic and equilibrium method results were in good agreement in all regions investigated. Iodine-125-iomazenil binding potential measured in vitro in 12 postmortem samples was found to be consistent with SPECT in vivo measurements. CONCLUSION: These studies demonstrated the feasibility of quantification of receptor binding with SPECT.
Authors: Anissa Abi-Dargham; Osama Mawlawi; Ilise Lombardo; Roberto Gil; Diana Martinez; Yiyun Huang; Dah-Ren Hwang; John Keilp; Lisa Kochan; Ronald Van Heertum; Jack M Gorman; Marc Laruelle Journal: J Neurosci Date: 2002-05-01 Impact factor: 6.167
Authors: Kelly P Cosgrove; Irina Esterlis; Graeme F Mason; Frederic Bois; Stephanie S O'Malley; John H Krystal Journal: Neuropharmacology Date: 2011-01-27 Impact factor: 5.250
Authors: Grant T Gullberg; Bryan W Reutter; Arkadiusz Sitek; Jonathan S Maltz; Thomas F Budinger Journal: Phys Med Biol Date: 2010-09-22 Impact factor: 3.609
Authors: Rajesh Narendran; N Scott Mason; Maureen A May; Chi-Min Chen; Steve Kendro; Khanum Ridler; Eugenii A Rabiner; Marc Laruelle; Chester A Mathis; W Gordon Frankle Journal: Synapse Date: 2011-01 Impact factor: 2.562
Authors: Alane S Kimes; Svetlana I Chefer; John A Matochik; Carlo S Contoreggi; D Bruce Vaupel; Elliot A Stein; Alexey G Mukhin Journal: Neuroimage Date: 2007-09-19 Impact factor: 6.556
Authors: A Soricelli; A Postiglione; M R Grivet-Fojaja; P P Mainenti; A Discepolo; A Varrone; M Salvatore; N A Lassen Journal: Eur J Nucl Med Date: 1996-10
Authors: Rajesh Narendran; Brian J Lopresti; Neale Scott Mason; Lora Deuitch; Jennifer Paris; Michael L Himes; Chowdari V Kodavali; Vishwajit L Nimgaonkar Journal: J Neurosci Date: 2014-07-23 Impact factor: 6.167