Literature DB >> 8294161

Nonenzymatic versus enzymatic hepatocyte isolation from pig livers for larger scale investigations of liver cell perfusion systems.

J C Gerlach1, J Brombacher, J M Courtney, P Neuhaus.   

Abstract

A comparison of nonenzymatic and enzymatic hepatocyte isolation was performed on pig livers. The collagenase perfusion showed superior results: mean viability 72 +/- 10% versus a maximum viability of 21% using EDTA-perfusion. A five-step collagenase perfusion technique, developed for pig livers enables larger scale investigations, in order to develop methods for hepatocyte cultures in therapeutical liver cell perfusion systems.

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Year:  1993        PMID： 8294161

Source DB:  PubMed          Journal:  Int J Artif Organs        ISSN： 0391-3988            Impact factor:   1.595


  6 in total

1.  Transplantation of hepatocytes from genetically engineered pigs into baboons.

Authors:  Hayato Iwase; Hong Liu; Eva Schmelzer; Mohamed Ezzelarab; Martin Wijkstrom; Hidetaka Hara; Whayoung Lee; Jagjit Singh; Cassandra Long; Eric Lagasse; Jörg C Gerlach; David K C Cooper; Bruno Gridelli
Journal:  Xenotransplantation       Date:  2017-01-28       Impact factor: 3.907

2.  [Addition of an osmotic agent to liver preservative solutions in a model of in vitro preservation of hepatocytes].

Authors:  K Klöppel; J Gerlach; P Neuhaus
Journal:  Langenbecks Arch Chir       Date:  1994

3.  Culture model for primary hepatocytes.

Authors:  J C Gerlach; P Neuhaus
Journal:  In Vitro Cell Dev Biol Anim       Date:  1994-10       Impact factor: 2.416

Review 4.  Experimental hepatocyte xenotransplantation--a comprehensive review of the literature.

Authors:  Huidong Zhou; Hong Liu; Mohamed Ezzelarab; Eva Schmelzer; Yi Wang; Jörg Gerlach; Bruno Gridelli; David K C Cooper
Journal:  Xenotransplantation       Date:  2015-05-07       Impact factor: 3.907

5.  [The effect of buffers in liver preservation solutions on hepatocytes in a model of in vitro preservation and reoxygenation].

Authors:  K Klöppel; J Gerlach; P Neuhaus
Journal:  Langenbecks Arch Chir       Date:  1994

6.  [The electrolyte composition of liver preservation solutions for hepatocytes in a model of in vitro preservation and reoxygenation].

Authors:  K Klöppel; J Gerlach; P Neuhaus
Journal:  Langenbecks Arch Chir       Date:  1994
  6 in total

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