Literature DB >> 8294034

New vectors for direct cloning of PCR products.

J Cha1, W Bishai, S Chandrasegaran.   

Abstract

We describe the construction of two new vectors for direct cloning of polymerase chain reaction (PCR) products. This was done by inserting a synthetic DNA fragment containing two adjacent XcmI sites between the Asp718 and BamHI sites of the M13mp18 and M13mp19 phages. Cleavage of these M13 derivatives with XcmI will result in a linearized vector with a single thymidine nucleotide at the 3' ends. Thus, these vectors would be very useful for direct cloning of PCR-generated products with high efficiency.

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Year:  1993        PMID： 8294034     DOI： 10.1016/0378-1119(93)90498-r

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  11 in total

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9.  Transgenic resistance by N gene of a Peanut bud necrosis virus isolate of characteristic phylogeny.

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10.  Construction of a High Efficiency PCR Products Cloning T Vector Using pGEM-5zf (+).

Authors:  Yaofeng Zhao; Zhancai Liu; Shuyang Yu; Sicheng Wen; Lennart Hammarstrom; Hodjattallah Rabbani
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