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Literature DB >> 8291195

Detection of Cryptosporidium parvum using a specific polymerase chain reaction.

K A Webster¹, J D Pow, M Giles, J Catchpole, M J Woodward.

Abstract

The design and use of polymerase chain reaction primers and probes as reagents for the detection of Cryptosporidium parvum are described. Sensitive and specific amplification of a 329 base pair product was demonstrated by ethidium bromide staining and hybridisation of radiolabelled probes. These reagents have the potential for application to diagnostic samples, environmental monitoring and epidemiological surveys.

Entities: Chemical Species

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Year: 1993 PMID： 8291195 DOI： 10.1016/0304-4017(93)90005-8

Source DB: PubMed Journal: Vet Parasitol ISSN： 0304-4017 Impact factor: 2.738

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Cited

18 in total

1. Immunomagnetic capture PCR to detect viable Cryptosporidium parvum oocysts from environmental samples.

Authors: M Q Deng; D O Cliver; T W Mariam
Journal: Appl Environ Microbiol Date: 1997-08 Impact factor: 4.792

2. Development of a novel, rapid integrated Cryptosporidium parvum detection assay.

Authors: D Kozwich; K A Johansen; K Landau; C A Roehl; S Woronoff; P A Roehl
Journal: Appl Environ Microbiol Date: 2000-07 Impact factor: 4.792

3. Failure to differentiate Cryptosporidium parvum from C. meleagridis based on PCR amplification of eight DNA sequences.

Authors: D Champliaud; P Gobet; M Naciri; O Vagner; J Lopez; J C Buisson; I Varga; G Harly; R Mancassola; A Bonnin
Journal: Appl Environ Microbiol Date: 1998-04 Impact factor: 4.792

4. PCR-restriction fragment length polymorphism analysis of a diagnostic 452-base-pair DNA fragment discriminates between Cryptosporidium parvum and C. meleagridis and between C. parvum isolates of human and animal origin.

Authors: K Guyot; A Follet-Dumoulin; C Recourt; E Lelièvre; J C Cailliez; E Dei-Cas
Journal: Appl Environ Microbiol Date: 2002-04 Impact factor: 4.792

5. Evaluation of Cryptosporidium parvum genotyping techniques.

Authors: I M Sulaiman; L Xiao; A A Lal
Journal: Appl Environ Microbiol Date: 1999-10 Impact factor: 4.792

6. An assay combining cell culture with reverse transcriptase PCR to detect and determine the infectivity of waterborne Cryptosporidium parvum.

Authors: P A Rochelle; D M Ferguson; T J Handojo; R De Leon; M H Stewart; R L Wolfe
Journal: Appl Environ Microbiol Date: 1997-05 Impact factor: 4.792

Detection of Cryptosporidium parvum using a specific polymerase chain reaction.

1. Immunomagnetic capture PCR to detect viable Cryptosporidium parvum oocysts from environmental samples.

2. Development of a novel, rapid integrated Cryptosporidium parvum detection assay.

3. Failure to differentiate Cryptosporidium parvum from C. meleagridis based on PCR amplification of eight DNA sequences.

4. PCR-restriction fragment length polymorphism analysis of a diagnostic 452-base-pair DNA fragment discriminates between Cryptosporidium parvum and C. meleagridis and between C. parvum isolates of human and animal origin.

5. Evaluation of Cryptosporidium parvum genotyping techniques.

6. An assay combining cell culture with reverse transcriptase PCR to detect and determine the infectivity of waterborne Cryptosporidium parvum.

7. Simplified method for recovery and PCR detection of Cryptosporidium DNA from bovine feces.

8. Detection of Cryptosporidium parvum DNA in human feces by nested PCR.

9. Phylogenetic analysis of Cryptosporidium parasites based on the small-subunit rRNA gene locus.

Review 10. Epidemiology and detection as options for control of viral and parasitic foodborne disease.