Literature DB >> 8288891

Development of an ELISA for measuring the activity of tetanus toxoid in vaccines and comparison with the toxin neutralization test in mice.

M A Manghi1, M F Pasetti, M L Brero, S Deluchi, G di Paola, V Mathet, P V Eriksson.   

Abstract

An enzyme-linked immunosorbent assay (ELISA) has been developed to measure anti-tetanus toxoid antibody levels in immunized guinea-pig sera as a useful alternative to the currently used toxin neutralization test (TNT) in determining the activity of the tetanus toxoid in vaccines. The ELISA was found to measure antibody levels as low as 5.8 x 10(-5) IU/ml. Furthermore, a comparison of the results from ELISA and TNT involving 132 different commercial vaccines showed a very good correlation (r = 0.94, p < 0.001) between antibody levels measured by both methods. The results suggest that the proposed ELISA is a reliable, simple and economical alternative to the TNT in mice for assessing the activity of tetanus toxoids in vaccines.

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Year:  1994        PMID： 8288891     DOI： 10.1016/0022-1759(94)90204-6

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  6 in total

1.  Neisseria meningitidis native outer membrane vesicles containing different lipopolysaccharide glycoforms as adjuvants for meningococcal and nonmeningococcal antigens.

Authors:  Jerry C Nagaputra; Christine S Rollier; Manish Sadarangani; J Claire Hoe; Ojas Hrakesh Mehta; Gunnstein Norheim; Muhammad Saleem; Hannah Chan; Jeremy P Derrick; Ian Feavers; Andrew J Pollard; E Richard Moxon
Journal:  Clin Vaccine Immunol       Date:  2013-12-18

2.  Serum antibodies to diphtheria-tetanus-pertussis vaccine components in Argentine children.

Authors:  M Pasetti; P Eriksson; F Ferrero; M Manghi
Journal:  Infection       Date:  1997 Nov-Dec       Impact factor: 3.553

3.  Immunogenicity and Reactogenicity following Primary Immunisation with a Combined DTaP-HBV Vaccine and a Haemophilus influenzae Type B Vaccine Administered by Separate or Mixed Injection.

Authors:  G Gabutti; G Bona; P Dentico; F Bamfi; K Hardt; S Majori; P Crovari
Journal:  Clin Drug Investig       Date:  2005       Impact factor: 2.859

4.  Tetanus immunization in pregnant women. Serum levels of antitetanus antibodies at time of delivery.

Authors:  I Maral; M Cirak; F N Aksakal; Z Baykan; F Kayikcioglu; M A Bumin
Journal:  Eur J Epidemiol       Date:  2001       Impact factor: 8.082

5.  Cross-Reactive Effects of Vaccines: Heterologous Immunity between Tetanus and Chlamydia.

Authors:  Marijana Stojanovic; Ivana Lukic; Emilija Marinkovic; Ana Kovacevic; Radmila Miljkovic; Joshua Tobias; Irma Schabussova; Mario Zlatović; Talin Barisani-Asenbauer; Ursula Wiedermann; Aleksandra Inic-Kanada
Journal:  Vaccines (Basel)       Date:  2020-12-01

6.  Vaccination response to tetanus toxoid and 23-valent pneumococcal vaccines following administration of a single dose of abatacept: a randomized, open-label, parallel group study in healthy subjects.

Authors:  Lee Tay; Francisco Leon; George Vratsanos; Ralph Raymond; Michael Corbo
Journal:  Arthritis Res Ther       Date:  2007       Impact factor: 5.156
  6 in total

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