DNA sequence analysis of human chromosome 21 notI linking clones.

Portions of 16 chromosome 21 NotI linking clones were sequenced. These linking clone sequences represent sequence-tagged restriction sites that are potentially useful for finding genes and for finer genome mapping and sequencing. All of the clones were G+C rich (54 to 83%). CpG and GpC dinucleotide frequencies were very close to the expected values based on base composition and were very similar in 15 of the clones. Most of the NotI linking clones were derived from CpG islands, which are often associated with genes. Five NotI linking clones had a high potential for coding regions; 7 additional clones may also contain coding regions. The NotI linking clones had many short homologous regions, but no extensive homologies either with each other or with GenBank sequences.