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Literature DB >> 8286360

Preferential binding of H1e histone to GC-rich DNA.

Abstract

We have investigated the binding of a pure H1 histone, the mouse variant H1e, to a 214 bp fragment of DNA from pBR322. Binding was monitored by observing the effects of the protein on melting of the DNA and by a gel-mobility-shift assay. We found, using this highly purified system, that H1e protein binds preferentially and cooperatively to the GC-rich region of the DNA. A chemically synthesized peptide containing 25 residues, corresponding to a region of the carboxyl-terminal domain of H1e, shows the same sequence preference but does not exhibit cooperativity.

Entities: Chemical Gene Species

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Year: 1994 PMID： 8286360 DOI： 10.1021/bi00167a049

Source DB: PubMed Journal: Biochemistry ISSN： 0006-2960 Impact factor: 3.162

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Cited

5 in total

1. Differential effect of H1 variant overproduction on gene expression is due to differences in the central globular domain.

Authors: D T Brown; A Gunjan; B T Alexander; D B Sittman
Journal: Nucleic Acids Res Date: 1997-12-15 Impact factor: 16.971

5. Temperature and osmotic stress dependence of the thermodynamics for binding linker histone H1⁰, Its carboxyl domain (H1⁰-C) or globular domain (H1⁰-G) to B-DNA.

Authors: V R Machha; C G Mikek; S Wellman; E A Lewis
Journal: Biochem Biophys Rep Date: 2017-10-13

5 in total

Preferential binding of H1e histone to GC-rich DNA.

1. Differential effect of H1 variant overproduction on gene expression is due to differences in the central globular domain.

2. High-affinity binding sites for histone H1 in plasmid DNA.

3. Differential association of linker histones H1 and H5 with telomeric nucleosomes in chicken erythrocytes.

4. Binding of histone H1e-c variants to CpG-rich DNA correlates with the inhibitory effect on enzymic DNA methylation.

5. Temperature and osmotic stress dependence of the thermodynamics for binding linker histone H1⁰, Its carboxyl domain (H1⁰-C) or globular domain (H1⁰-G) to B-DNA.

Preferential binding of H1e histone to GC-rich DNA.

1. Differential effect of H1 variant overproduction on gene expression is due to differences in the central globular domain.

2. High-affinity binding sites for histone H1 in plasmid DNA.

3. Differential association of linker histones H1 and H5 with telomeric nucleosomes in chicken erythrocytes.

4. Binding of histone H1e-c variants to CpG-rich DNA correlates with the inhibitory effect on enzymic DNA methylation.

5. Temperature and osmotic stress dependence of the thermodynamics for binding linker histone H10, Its carboxyl domain (H10-C) or globular domain (H10-G) to B-DNA.

5. Temperature and osmotic stress dependence of the thermodynamics for binding linker histone H1⁰, Its carboxyl domain (H1⁰-C) or globular domain (H1⁰-G) to B-DNA.