Literature DB >> 8285693

Secretion of streptavidin from Bacillus subtilis.

V Nagarajan1, R Ramaley, H Albertson, M Chen.   

Abstract

Streptavidin is an extracellular tetrameric protein produced by Streptomyces avidinii. A series of hybrid gene fusions consisting of Bacillus signal peptide coding regions fused to the mature streptavidin sequence were constructed. B. subtilis strains harboring these plasmids accumulate a tetrameric streptavidin in the growth medium. The properties of the streptavidin produced by B. subtilis are similar to those of the streptavidin produced by S. avidinii. B. subtilis strains carrying the various fusions can be grown to a high cell density in a biotin-free medium. Thus, B. subtilis represents an alternate host system for the production of streptavidin.

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Year:  1993        PMID： 8285693      PMCID： PMC182546          DOI： 10.1128/aem.59.11.3894-3898.1993

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  12 in total

Review 1.  Introduction to avidin-biotin technology.

Authors:  M Wilchek; E A Bayer
Journal:  Methods Enzymol       Date:  1990       Impact factor: 1.600

2.  System for secretion of heterologous proteins in Bacillus subtilis.

Authors:  V Nagarajan
Journal:  Methods Enzymol       Date:  1990       Impact factor: 1.600

3.  Modular expression and secretion vectors for Bacillus subtilis.

Authors:  V Nagarajan; H Albertson; M Chen; J Ribbe
Journal:  Gene       Date:  1992-05-01       Impact factor: 3.688

4.  Single-step purification of avidine from egg white by affinity chromatography on biocytin-Sepharose columns.

Authors:  P Cuatrecasas; M Wilchek
Journal:  Biochem Biophys Res Commun       Date:  1968-10-24       Impact factor: 3.575

5.  Structural origins of high-affinity biotin binding to streptavidin.

Authors:  P C Weber; D H Ohlendorf; J J Wendoloski; F R Salemme
Journal:  Science       Date:  1989-01-06       Impact factor: 47.728

6.  Expression of a cloned streptavidin gene in Escherichia coli.

Authors:  T Sano; C R Cantor
Journal:  Proc Natl Acad Sci U S A       Date:  1990-01       Impact factor: 11.205

Review 7.  Protein secretion in Bacillus species.

Authors:  M Simonen; I Palva
Journal:  Microbiol Rev       Date:  1993-03

8.  The purification of avidin and its derivatives on 2-iminobiotin-6-aminohexyl-Sepharose 4B.

Authors:  G Heney; G A Orr
Journal:  Anal Biochem       Date:  1981-06       Impact factor: 3.365

9.  Expression vectors for streptavidin-containing chimeric proteins.

Authors:  T Sano; C R Cantor
Journal:  Biochem Biophys Res Commun       Date:  1991-04-30       Impact factor: 3.575

10.  Iminobiotin affinity columns and their application to retrieval of streptavidin.

Authors:  K Hofmann; S W Wood; C C Brinton; J A Montibeller; F M Finn
Journal:  Proc Natl Acad Sci U S A       Date:  1980-08       Impact factor: 11.205
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  3 in total

1.  Reduction of growth and acetyl-CoA carboxylase activity by expression of a chimeric streptavidin gene in Escherichia coli.

Authors:  X Guan; E S Wurtele
Journal:  Appl Microbiol Biotechnol       Date:  1996-02       Impact factor: 4.813

2.  Engineering of a Bacillus subtilis strain with adjustable levels of intracellular biotin for secretory production of functional streptavidin.

Authors:  Sau-Ching Wu; Sui-Lam Wong
Journal:  Appl Environ Microbiol       Date:  2002-03       Impact factor: 4.792

3.  Efficient production of active chicken avidin using a bacterial signal peptide in Escherichia coli.

Authors:  Vesa P Hytönen; Olli H Laitinen; Tomi T Airenne; Heidi Kidron; Niko J Meltola; Eevaleena J Porkka; Jarno Hörhä; Tiina Paldanius; Juha A E Määttä; Henri R Nordlund; Mark S Johnson; Tiina A Salminen; Kari J Airenne; Seppo Ylä-Herttuala; Markku S Kulomaa
Journal:  Biochem J       Date:  2004-12-01       Impact factor: 3.857
  3 in total

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