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Literature DB >> 8282201

Selection of human anti-hapten antibodies from semisynthetic libraries.

C F Barbas¹, W Amberg, A Simoncsits, T M Jones, R A Lerner.

Abstract

Semisynthetic human Fab libraries were constructed, displayed on the surface of filamentous phage and selected for binding to three hapten conjugates. A number of Fabs were isolated and characterized with respect to affinity and specificity. Fabs exhibited affinities of between 80 and 29 nM, as determined by surface plasmon resonance, for the conjugate on which they were selected. Conservation of Asp101 in the third heavy-chain complementarity determining region (HCDR3) appears to be important in the construction of synthetically diverse repertoires.

Entities: Chemical Gene Species

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Year: 1993 PMID： 8282201 DOI： 10.1016/0378-1119(93)90251-w

Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688

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Cited

8 in total

Review 1. Generation of recombinant antibodies.

Authors: S M Kipriyanov; M Little
Journal: Mol Biotechnol Date: 1999-09 Impact factor: 2.695

2. Rolling replication of short DNA circles.

Authors: A Fire; S Q Xu
Journal: Proc Natl Acad Sci U S A Date: 1995-05-09 Impact factor: 11.205

3. In vitro evolution of a neutralizing human antibody to human immunodeficiency virus type 1 to enhance affinity and broaden strain cross-reactivity.

Authors: C F Barbas; D Hu; N Dunlop; L Sawyer; D Cababa; R M Hendry; P L Nara; D R Burton
Journal: Proc Natl Acad Sci U S A Date: 1994-04-26 Impact factor: 11.205

Selection of human anti-hapten antibodies from semisynthetic libraries.

Review 1. Generation of recombinant antibodies.

2. Rolling replication of short DNA circles.

3. In vitro evolution of a neutralizing human antibody to human immunodeficiency virus type 1 to enhance affinity and broaden strain cross-reactivity.

Review 4. The importance of being tyrosine: lessons in molecular recognition from minimalist synthetic binding proteins.

5. Natural and man-made V-gene repertoires for antibody discovery.

6. Selection of diethylstilbestrol-specific single-chain antibodies from a non-immunized mouse ribosome display library.

7. Quantitative PCR is a Valuable Tool to Monitor the Performance of DNA-Encoded Chemical Library Selections.

8. Isolation of high affinity human antibodies directly from large synthetic repertoires.