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Literature DB >> 8282124

The human sperm protein PH-20 has hyaluronidase activity.

Abstract

The PH-20 protein present on the membrane of guinea pig sperm was characterized using a monoclonal antibody [(1991) J. Cell Biol. 111, 2939-2949]. We have isolated the cDNA encoding the human PH-20 protein from a testis library. This cDNA was expressed in RK 13 cells using a vaccinia virus expression system. Cells expressing the human PH-20 protein possess hyaluronidase activity. Treatment with PI-PLC releases the hyaluronidase into the the medium with a concomitant large increase in enzymatic activity. These results demonstrate that the human PH-20 protein has hyaluronidase activity.

Entities: Gene Species

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Year: 1993 PMID： 8282124 DOI： 10.1016/0014-5793(93)80873-s

Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124

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Cited

26 in total

1. A pRb-responsive, RGD-modified, and hyaluronidase-armed canine oncolytic adenovirus for application in veterinary oncology.

Authors: Eduardo Laborda; Cristina Puig-Saus; Alba Rodriguez-García; Rafael Moreno; Manel Cascalló; Josep Pastor; Ramon Alemany
Journal: Mol Ther Date: 2014-01-22 Impact factor: 11.454

2. Release of the glycosylphosphatidylinositol-anchored enzyme ecto-5'-nucleotidase by phospholipase C: catalytic activation and modulation by the lipid bilayer.

Authors: M T Lehto; F J Sharom
Journal: Biochem J Date: 1998-05-15 Impact factor: 3.857

Review 3. Hyaluronidases--a group of neglected enzymes.

Authors: G Kreil
Journal: Protein Sci Date: 1995-09 Impact factor: 6.725

4. PH-20 and sperm hyaluronidase: a conceptual conundrum in mammalian fertilization.

Authors: P Gacesa; N D Civill; R A Harrison
Journal: Biochem J Date: 1994-10-01 Impact factor: 3.857

Review 5. Immunologic roles of hyaluronan.

Authors: Mark E Mummert
Journal: Immunol Res Date: 2005 Impact factor: 2.829

6. Mutations in HYAL1, a member of a tandemly distributed multigene family encoding disparate hyaluronidase activities, cause a newly described lysosomal disorder, mucopolysaccharidosis IX.

Authors: B Triggs-Raine; T J Salo; H Zhang; B A Wicklow; M R Natowicz
Journal: Proc Natl Acad Sci U S A Date: 1999-05-25 Impact factor: 11.205

The human sperm protein PH-20 has hyaluronidase activity.

1. A pRb-responsive, RGD-modified, and hyaluronidase-armed canine oncolytic adenovirus for application in veterinary oncology.

2. Release of the glycosylphosphatidylinositol-anchored enzyme ecto-5'-nucleotidase by phospholipase C: catalytic activation and modulation by the lipid bilayer.

Review 3. Hyaluronidases--a group of neglected enzymes.

4. PH-20 and sperm hyaluronidase: a conceptual conundrum in mammalian fertilization.

Review 5. Immunologic roles of hyaluronan.

6. Mutations in HYAL1, a member of a tandemly distributed multigene family encoding disparate hyaluronidase activities, cause a newly described lysosomal disorder, mucopolysaccharidosis IX.

7. Cells expressing the DG42 gene from early Xenopus embryos synthesize hyaluronan.

Review 8. Biology of hyaluronan: Insights from genetic disorders of hyaluronan metabolism.

Review 9. The molecular basis of gamete recognition in mice and humans.

10. Elucidating the processes and pathways enriched in buffalo sperm proteome in regulating semen quality.