| Literature DB >> 8280777 |
J D Morrow1, T A Minton, K F Badr, L J Roberts.
Abstract
F2-isoprostanes are prostaglandin (PG)F2-like compounds that are produced in vivo as non-enzymatic products of free radical catalyzed peroxidation of arachidonic acid. One F2-isoprostane whose formation should be favored is 8-epi-PGF2 alpha. 8-Epi-PGF2 alpha has been shown to exert potent bioactivity but proof that it is formed in vivo is lacking. Evidence is now presented suggesting that 8-epi-PGF2 alpha is, in fact, formed in vivo by demonstrating that an endogenous F2-isoprostane with a retention time on capillary GC identical with that of 8-epi-PGF2 alpha co-chromatographs through four high resolving HPLC purification procedures with authentic radiolabelled 8-epi-PGF2 alpha.Entities:
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Year: 1994 PMID： 8280777 DOI： 10.1016/0005-2760(94)90128-7
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002