Literature DB >> 8278492

Expression of Escherichia coli phosphoenolpyruvate carboxylase in a cyanobacterium. Functional complementation of Synechococcus PCC 7942 ppc.

I Luinenburg1, J R Coleman.   

Abstract

The gene (ppc) coding for phosphoenolpyruvate carboxylase (PEPCase) in the cyanobacterium Synechococcus PCC 7942 has been inactivated via insertional mutagenesis while being functionally complemented by Escherichia coli ppc. Cyanobacterial cells functionally complemented by E. coli ppc showed decreased PEPCase activity in crude cell lysates and detergent-permeabilized whole cell assays. Decreased rates of growth, reduced levels of chlorophyll a, and decreased photosynthetic O2 evolution capacity per cell when compared to wild-type cyanobacterial cells were also observed. Phycobiliprotein levels were not affected. The results are discussed in terms of the impact of reduced PEPCase activity on cyanobacterial cell metabolism and the regulatory properties of the E. coli gene product.

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Year:  1993        PMID： 8278492      PMCID： PMC158655          DOI： 10.1104/pp.101.1.121

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  13 in total

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Journal:  J Biol Chem       Date:  1966-05-25       Impact factor: 5.157

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Journal:  J Biol Chem       Date:  1980-02-25       Impact factor: 5.157

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Journal:  Arch Microbiol       Date:  1978-03       Impact factor: 2.552

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Authors:  S Gendel; N Straus; D Pulleyblank; J Williams
Journal:  J Bacteriol       Date:  1983-10       Impact factor: 3.490

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Authors:  H Sabe; T Miwa; T Kodaki; K Izui; S Hiraga; H Katsuki
Journal:  Gene       Date:  1984-11       Impact factor: 3.688

10.  Expression of a family of psbA genes encoding a photosystem II polypeptide in the cyanobacterium Anacystis nidulans R2.

Authors:  S S Golden; J Brusslan; R Haselkorn
Journal:  EMBO J       Date:  1986-11       Impact factor: 11.598
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  5 in total

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4.  Systematic identification and elimination of flux bottlenecks in the aldehyde production pathway of Synechococcus elongatus PCC 7942.

Authors:  Yi Ern Cheah; Yao Xu; Sarah A Sacco; Piyoosh K Babele; Amy O Zheng; Carl Hirschie Johnson; Jamey D Young
Journal:  Metab Eng       Date:  2020-03-25       Impact factor: 9.783

5.  Application of synthetic biology in cyanobacteria and algae.

Authors:  Bo Wang; Jiangxin Wang; Weiwen Zhang; Deirdre R Meldrum
Journal:  Front Microbiol       Date:  2012-09-19       Impact factor: 5.640
  5 in total

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