| Literature DB >> 8275506 |
F J Kormelink1, R A Hoffmann, H Gruppen, A G Voragen, J P Kamerling, J F Vliegenthart.
Abstract
Alkali-extractable wheat-flour arabinoxylan, treated with endo-(1-->4)-beta-D-xylanase III from Aspergillus awamori CMI 142717, was fractionated by Bio-Gel P-2 size exclusion chromatography at 60 degrees C. Column fractions, corresponding to oligosaccharides with degrees of polymerisation from 5 to 10, were collected, and subfractionated by high performance anion-exchange chromatography on CarboPac PA-1. The structures of the oligosaccharides thus obtained were elucidated by 1H NMR spectroscopy, showing chains of (1-->4)-linked beta-D-xylopyranosyl residues differently substituted at O-3 and/or O-2,3 with alpha-L-arabinofuranosyl groups. The structures were different from those obtained with endo-(1-->4)-beta-D-xylanase I of the same xylanolytic enzyme system.Entities:
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Year: 1993 PMID： 8275506 DOI： 10.1016/0008-6215(93)84101-b
Source DB: PubMed Journal: Carbohydr Res ISSN： 0008-6215 Impact factor: 2.104