Literature DB >> 827428

Distribution of RNA polymerase on Drosophila polytene chromosomes as studied by indirect immunofluorescence.

U Plagens, A L Greenleaf, E K Bautz.   

Abstract

Using indirect immunofluorescence visualization techniques we investigated the in situ distribution of RNA polymerase B on Drosophila melanogaster polytene chromosomes. The enzyme was found at many sites distributed throughout the genome in a pattern clearly distinct from that observed for histone H1, but it was especially concentrated in puffs induced by heat shock.

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Year:  1976        PMID： 827428     DOI： 10.1007/BF00328484

Source DB:  PubMed          Journal:  Chromosoma        ISSN： 0009-5915            Impact factor:   4.316


  18 in total

Review 1.  Eukaryotic nuclear RNA polymerases.

Authors:  P Chambon
Journal:  Annu Rev Biochem       Date:  1975       Impact factor: 23.643

2.  Effect of alpha-amanitin on chromosomal and nucleolar RNA-synthesis in Chironomus thummi polytene chromosomes.

Authors:  E Serfling; U Wobus; R Panitz
Journal:  FEBS Lett       Date:  1972-02-01       Impact factor: 4.124

3.  [RIBONUCLEIC ACID SYNTHESIS IN GIANT CHROMOSOMES. AUTORADIOGRAPHIC INVESTIGATIONS ON CHIRONOMUS TENTANS].

Authors:  C PELLING
Journal:  Chromosoma       Date:  1964-04-01       Impact factor: 4.316

4.  Micrurgical studies on Chironomus salivary gland chromosomes.

Authors:  E G D'ANGELO
Journal:  Biol Bull       Date:  1946-02       Impact factor: 1.818

5.  Distinct molecular structures of nuclear class I, II, and III DNA-dependent RNA polymerases.

Authors:  V E Sklar; L B Schwartz; R G Roeder
Journal:  Proc Natl Acad Sci U S A       Date:  1975-01       Impact factor: 11.205

6.  Histones of polytene and nonpolytene nuclei of Drosophila melanogaster.

Authors:  L H Cohen; B V Gotchel
Journal:  J Biol Chem       Date:  1971-03-25       Impact factor: 5.157

7.  A method for determination of the in situ distribution of chromosomal proteins.

Authors:  L M Silver; S C Elgin
Journal:  Proc Natl Acad Sci U S A       Date:  1976-02       Impact factor: 11.205

8.  Histones in fixed cytological preparations of Chinese hamster chromosomes demonstrated by immunofluorescence.

Authors:  L Pothier; J F Gallagher; C E Wright; P R Libby
Journal:  Nature       Date:  1975-05-22       Impact factor: 49.962

9.  Immunofluorescent labeling of chromosomes with antisera to histones and histone fractions.

Authors:  L S Desai; L Pothier; G E Foley; R A Adams
Journal:  Exp Cell Res       Date:  1972-02       Impact factor: 3.905

10.  Effects of -amanitin on in vitro labeling of RNA from defined nuclear components in salivary gland cells from Chironomus tentans.

Authors:  E Egyházi; B D'Monte; J E Edström
Journal:  J Cell Biol       Date:  1972-05       Impact factor: 10.539
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  30 in total

Review 1.  Polytene chromosome squash methods for studying transcription and epigenetic chromatin modification in Drosophila using antibodies.

Authors:  Kristen M Johansen; Weili Cai; Huai Deng; Xiaomin Bao; Weiguo Zhang; Jack Girton; Jørgen Johansen
Journal:  Methods       Date:  2009-03-09       Impact factor: 3.608

2.  Discrimination of potential and actual RNA polymerase B activity in isolated nuclei during differentiation ofPhysarum polycephalum.

Authors:  Armin Hildebrandt; Helmut W Sauer
Journal:  Wilehm Roux Arch Dev Biol       Date:  1977-06

3.  The presence of an antigen reactive with a human autoantibody in Trichosia pubescens (Diptera: Sciaridae) and its association with certain transcriptionally active regions of the genome.

Authors:  J M Amabis; D C Amabis; J Kaburaki; B D Stollar
Journal:  Chromosoma       Date:  1990-04       Impact factor: 4.316

4.  Isolation and distribution of a Drosophila protein preferentially associated with active regions of the genome.

Authors:  B Fleischmann; R Filipski; G Fleischmann
Journal:  Chromosoma       Date:  1989-03       Impact factor: 4.316

5.  A cytological and molecular analysis of Adh gene expression in Drosophila melanogaster polytene chromosomes.

Authors:  N Visa; R Gonzàlez-Duarte; M C Santa-Cruz
Journal:  Chromosoma       Date:  1988       Impact factor: 4.316

6.  Production and characterization of antisera against three individual NHC proteins; a case of a generally distributed NHC protein.

Authors:  L M Silver; S C Elgin
Journal:  Chromosoma       Date:  1978-08-28       Impact factor: 4.316

7.  Correlation of RNA polymerase B and transcriptional activity in the chromosomes of Drosophila melanogaster.

Authors:  M Jamrich; R Haars; E Wulf; F A Bautz
Journal:  Chromosoma       Date:  1977-12-06       Impact factor: 4.316

8.  Drosophila ELL is associated with actively elongating RNA polymerase II on transcriptionally active sites in vivo.

Authors:  M Gerber; J Ma; K Dean; J C Eissenberg; A Shilatifard
Journal:  EMBO J       Date:  2001-11-01       Impact factor: 11.598

9.  Isolation and distribution of a Drosophila protein preferentially associated with inactive regions of the genome.

Authors:  G Fleischmann; R Filipski; S C Elgin
Journal:  Chromosoma       Date:  1987       Impact factor: 4.316

10.  Effect of salt-treatment on manually isolated polytene chromosomes from Chironomus tentans.

Authors:  U Plagens
Journal:  Chromosoma       Date:  1978-08-21       Impact factor: 4.316
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