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Literature DB >> 8270005

A quantitative polymerase chain reaction method for the detection in avian faeces of salmonellas carrying the spvR gene.

Abstract

A quick, semi-quantitative method of detecting Salmonella species which contain the virulence plasmid has been developed using the polymerase chain reaction (PCR). A pair of primers have been synthesized encompassing a 500 bp fragment of the spvR virulence gene. Competitor DNA consisting of the spvR gene with a 94 bp deletion situated between the primer recognition sequences, was cloned into a plasmid vector. Co-amplification of the 'unknown' target salmonella DNA with known quantities of competitor DNA in the same reaction tube gave PCR products of 500 and 406 bp respectively. Visual assessment of the ratio of the two products on ethidium bromide stained agarose gels provided an estimate of the approximate number of salmonella cells present in avian faeces. The technique could be applied to detect quantifiably any non-host DNA in clinical samples if a suitable DNA sequence for primer construction is available.

Entities: Chemical Species

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Year: 1993 PMID： 8270005 PMCID： PMC2271269 DOI： 10.1017/s0950268800057186

Source DB: PubMed Journal: Epidemiol Infect ISSN： 0950-2688 Impact factor: 2.451

25 in total

1. Inhibitory effects of urine on the polymerase chain reaction for cytomegalovirus DNA.

Authors: G Khan; H O Kangro; P J Coates; R B Heath
Journal: J Clin Pathol Date: 1991-05 Impact factor: 3.411

2. Rapid fluorescence method for screening Salmonella spp. from enteric differential agars.

Authors: P M Aguirre; J B Cacho; L Folgueira; M López; J García; A C Velasco
Journal: J Clin Microbiol Date: 1990-01 Impact factor: 5.948

3. Detection of human immunodeficiency virus type 1 in AIDS patients using amplification-mediated hybridization analyses: reproducibility and quantitative limitations.

Authors: G R Davis; K Blumeyer; L J DiMichele; K M Whitfield; H Chappelle; N Riggs; S S Ghosh; P M Kao; E Fahy; D Y Kwoh
Journal: J Infect Dis Date: 1990-07 Impact factor: 5.226

4. Detection and quantification of human immunodeficiency virus RNA in patient serum by use of the polymerase chain reaction.

Authors: M Holodniy; D A Katzenstein; S Sengupta; A M Wang; C Casipit; D H Schwartz; M Konrad; E Groves; T C Merigan
Journal: J Infect Dis Date: 1991-04 Impact factor: 5.226

5. Isolation of the replication and partitioning regions of the Salmonella typhimurium virulence plasmid and stabilization of heterologous replicons.

Authors: S A Tinge; R Curtiss
Journal: J Bacteriol Date: 1990-09 Impact factor: 3.490

6. Polymerase chain reaction for detection of adenoviruses in stool samples.

Authors: A Allard; R Girones; P Juto; G Wadell
Journal: J Clin Microbiol Date: 1990-12 Impact factor: 5.948

7. Detection of Shigella in feces using DNA amplification.

Authors: G Frankel; L Riley; J A Giron; J Valmassoi; A Friedmann; N Strockbine; S Falkow; G K Schoolnik
Journal: J Infect Dis Date: 1990-06 Impact factor: 5.226

8. Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction.

Authors: G Gilliland; S Perrin; K Blanchard; H F Bunn
Journal: Proc Natl Acad Sci U S A Date: 1990-04 Impact factor: 11.205

9. Use of the polymerase chain reaction for direct detection of Listeria monocytogenes in soft cheese.

Authors: K Wernars; C J Heuvelman; T Chakraborty; S H Notermans
Journal: J Appl Bacteriol Date: 1991-02

10. Quantitation of mRNA by the polymerase chain reaction.

Authors: A M Wang; M V Doyle; D F Mark
Journal: Proc Natl Acad Sci U S A Date: 1989-12 Impact factor: 11.205

11 in total

1. PCR detection of Salmonella enterica serotype Montevideo in and on raw tomatoes using primers derived from hilA.

Authors: X Guo; J Chen; L R Beuchat; R E Brackett
Journal: Appl Environ Microbiol Date: 2000-12 Impact factor: 4.792

2. Detection of salmonellae in chicken feces by a combination of tetrathionate broth enrichment, capillary PCR, and capillary gel electrophoresis.

Authors: K T Carli; C B Unal; V Caner; A Eyigor
Journal: J Clin Microbiol Date: 2001-05 Impact factor: 5.948

10. Culture versus PCR for Salmonella Species Identification in Some Dairy Products and Dairy Handlers with Special Concern to Its Zoonotic Importance.

Authors: Mayada M Gwida; Maha A M Al-Ashmawy
Journal: Vet Med Int Date: 2014-04-03

A quantitative polymerase chain reaction method for the detection in avian faeces of salmonellas carrying the spvR gene.

1. Inhibitory effects of urine on the polymerase chain reaction for cytomegalovirus DNA.

2. Rapid fluorescence method for screening Salmonella spp. from enteric differential agars.

3. Detection of human immunodeficiency virus type 1 in AIDS patients using amplification-mediated hybridization analyses: reproducibility and quantitative limitations.

4. Detection and quantification of human immunodeficiency virus RNA in patient serum by use of the polymerase chain reaction.

5. Isolation of the replication and partitioning regions of the Salmonella typhimurium virulence plasmid and stabilization of heterologous replicons.

6. Polymerase chain reaction for detection of adenoviruses in stool samples.

7. Detection of Shigella in feces using DNA amplification.

8. Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction.

9. Use of the polymerase chain reaction for direct detection of Listeria monocytogenes in soft cheese.

10. Quantitation of mRNA by the polymerase chain reaction.

1. PCR detection of Salmonella enterica serotype Montevideo in and on raw tomatoes using primers derived from hilA.

2. Detection of salmonellae in chicken feces by a combination of tetrathionate broth enrichment, capillary PCR, and capillary gel electrophoresis.

3. Mining and evaluation of new specific molecular targets for the PCR detection of Salmonella spp. genome.

Review 4. Evolution of host adaptation in Salmonella enterica.

5. Simple and rapid detection of Salmonella by direct PCR amplification of gene fimW.

6. Synergistic effect of mutations in invA and lpfC on the ability of Salmonella typhimurium to cause murine typhoid.

7. Virulent Salmonella typhimurium has two periplasmic Cu, Zn-superoxide dismutases.

8. Rapid detection of Salmonella enterica with primers specific for iroB.

Review 9. Detection of animal pathogens by using the polymerase chain reaction (PCR).

10. Culture versus PCR for Salmonella Species Identification in Some Dairy Products and Dairy Handlers with Special Concern to Its Zoonotic Importance.