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Literature DB >> 8267983

Generation and identification of recombinant adenovirus by liposome-mediated transfection and PCR analysis.

W W Zhang¹, X Fang, C D Branch, W Mazur, B A French, J A Roth.

Abstract

A simplified method for generating recombinant adenovirus was developed by using liposome-mediated co-transfection and by directly observing for the cytopathic effect caused by recombinant adenovirus in transfected 293 cells. This approach avoided difficulties associated with calcium-phosphate precipitation and agarose overlays for plaque assays. The ease of generating recombinant adenovirus was considerably improved. Analysis by PCR of DNA samples from the supernatant of the cell cultures with the cytopathic effect was also developed, which made identification of any newly generated recombinant virus rapid and specific.

Entities: Chemical

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Year: 1993 PMID： 8267983

Source DB: PubMed Journal: Biotechniques ISSN： 0736-6205 Impact factor: 1.993

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Cited

14 in total

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2. Differential gene expression in p53-mediated apoptosis-resistant vs. apoptosis-sensitive tumor cell lines.

Authors: S A Maxwell; G E Davis
Journal: Proc Natl Acad Sci U S A Date: 2000-11-21 Impact factor: 11.205

3. Neurofilaments are transported rapidly but intermittently in axons: implications for slow axonal transport.

Authors: S Roy; P Coffee; G Smith; R K Liem; S T Brady; M M Black
Journal: J Neurosci Date: 2000-09-15 Impact factor: 6.167

4. Mitochondrial imaging in dorsal root ganglion neurons following the application of inducible adenoviral vector expressing two fluorescent proteins.

Authors: Payman Nasr; Patrick G Sullivan; George M Smith
Journal: J Neurosci Methods Date: 2008-05-03 Impact factor: 2.390

10. A helper-independent adenovirus vector with E1, E3, and fiber deleted: structure and infectivity of fiberless particles.

Authors: D J Von Seggern; C Y Chiu; S K Fleck; P L Stewart; G R Nemerow
Journal: J Virol Date: 1999-02 Impact factor: 5.103

Generation and identification of recombinant adenovirus by liposome-mediated transfection and PCR analysis.

Review 1. Recent advances in molecular biological techniques and their relevance to pulmonary research.

2. Differential gene expression in p53-mediated apoptosis-resistant vs. apoptosis-sensitive tumor cell lines.

3. Neurofilaments are transported rapidly but intermittently in axons: implications for slow axonal transport.

4. Mitochondrial imaging in dorsal root ganglion neurons following the application of inducible adenoviral vector expressing two fluorescent proteins.

5. Wild-type human p53 and a temperature-sensitive mutant induce Fas/APO-1 expression.

6. Bax involvement in p53-mediated neuronal cell death.

Review 7. Antisense oncogene and tumor suppressor gene therapy of cancer.

8. Contribution of p53-dependent caspase activation to neuronal cell death declines with neuronal maturation.

9. Efficient directional cloning of recombinant adenovirus vectors using DNA-protein complex.

10. A helper-independent adenovirus vector with E1, E3, and fiber deleted: structure and infectivity of fiberless particles.