Literature DB >> 826587

Radioimmunoassay for antibodies against Brucella abortus: a new serological test for bovine brucellosis.

R J Chappel, P Williamson, D J McNaught, M J Dalling, G S Allan.   

Abstract

A radioimmunoassay (RIA) has been developed to measure antibodies against Brucella abortus in bovine serum and can be used in the diagnosis of bovine brucellosis. The RIA measures the amount of specific antibody of the IgG1 and IgG2 subclasses but is insensitive to IgM, a characteristic which may make it more suitable than the complement fixation test (CFT) or the serum agglutination test for distinguishing infected animals from those which have been vaccinated with Br. abortus strain 19. The RIA is not subject to prozoning or ambiguous reactions, both of which interfere with the interpretation of the CFT.

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Year:  1976        PMID： 826587      PMCID： PMC2129824          DOI： 10.1017/s0022172400055741

Source DB:  PubMed          Journal:  J Hyg (Lond)        ISSN： 0022-1724


  8 in total

1.  The effect of natural infection with Salmonella urbana on the serological status of cattle in relation to tests for brucellosis.

Authors:  M J Corbel; C Wray
Journal:  Br Vet J       Date:  1975 May-Jun

2.  Studies on a nonspecific Brucella-agglutinating substance in bovin serum. I. The differentiation of the specific and nonspecific agglutinins by heat treatment.

Authors:  W R HESS
Journal:  Am J Vet Res       Date:  1953-04       Impact factor: 1.156

3.  A quantitative comparison of the sensitivity of serological test for bovine brucellosis to different antibody classes.

Authors:  G S Allan; R J Chappel; P Williamson; D J McNaught
Journal:  J Hyg (Lond)       Date:  1976-04

4.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

Review 5.  Immunity to brucella infection.

Authors:  S S Elberg
Journal:  Medicine (Baltimore)       Date:  1973-07       Impact factor: 1.889

6.  An enzymic method for the trace iodination of immunoglobulins and other proteins.

Authors:  J J Marchalonis
Journal:  Biochem J       Date:  1969-06       Impact factor: 3.857

7.  A mechanism for prozone formation in the complement fixation test for bovine brucellosis.

Authors:  P Plackett; G G Alton
Journal:  Aust Vet J       Date:  1975-08       Impact factor: 1.281

8.  The serological diagnosis of bovine brucellosis: an evaluation of the complement fixation, serum agglutination and rose bengal tests.

Authors:  G G Alton; J Maw; B A Rogerson; G G McPherson
Journal:  Aust Vet J       Date:  1975-02       Impact factor: 1.281
  8 in total
  5 in total

1.  The diagnostic efficiency of some serological tests for bovine brucellosis.

Authors:  R J Chappel; D J McNaught; J A Bourke; G S Allan
Journal:  J Hyg (Lond)       Date:  1978-06

2.  Comparison of the results of some serological tests for bovine brucellosis.

Authors:  R J Chappel; D J McNaught; J A Bourke; G S Allan
Journal:  J Hyg (Lond)       Date:  1978-06

3.  A modified radioimmunoassay for antibodies against Brucella abortus.

Authors:  R J Chappel; J Hayes; G J Brain; D J McNaught
Journal:  J Hyg (Lond)       Date:  1982-02

4.  A comparison of the results of the brucellosis radioimmunoassay and other serological test in experimentally infected cattle.

Authors:  J Hayes; R J Chappel
Journal:  J Hyg (Lond)       Date:  1982-02

5.  The serological response of cattle to vaccines against brucellosis, as measured by the brucellosis radioimmunoassay and other tests.

Authors:  R J Chappel; J Hayes; B A Rogerson; L J Shenfield
Journal:  J Hyg (Lond)       Date:  1982-02
  5 in total

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