Literature DB >> 8265352

Isolation of U3 snoRNP from CHO cells: a novel 55 kDa protein binds to the central part of U3 snoRNA.

B Lübben1, C Marshallsay, N Rottmann, R Lührmann.   

Abstract

U3 snoRNP, the most abundant of the small nucleolar ribonucleoprotein particles (snoRNPs), has previously been demonstrated to participate in pre-rRNA maturation. Here we report the purification of U3 snoRNP from CHO cells using anti-m3G-immunoaffinity and mono Q anion-exchange chromatography. Isolated U3 snoRNPs contain three novel proteins, of 15, 50 and 55 kDa respectively. These proteins may represent core U3 snoRNP proteins whose binding mediates the association of other proteins, such as fibrillarin, that are lost during purification. Using a rabbit antiserum raised against the 55 kDa protein, and an in vitro reconstitution assay, we have localised the 55 kDa protein binding site on the U3 snoRNA. Stable binding of the 55 kDa protein requires sequences located between nucleotides 97 and 204 of the human U3 snoRNA, including the evolutionarily conserved B and C sequence motifs.

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Year:  1993        PMID： 8265352      PMCID： PMC310574          DOI： 10.1093/nar/21.23.5377

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  29 in total

Review 1.  Structure and function of nucleolar snRNPs.

Authors:  W Filipowicz; T Kiss
Journal:  Mol Biol Rep       Date:  1993-08       Impact factor: 2.316

2.  Structural analysis of the human U3 ribonucleoprotein particle reveal a conserved sequence available for base pairing with pre-rRNA.

Authors:  K A Parker; J A Steitz
Journal:  Mol Cell Biol       Date:  1987-08       Impact factor: 4.272

3.  Maturation of a 100 kDa protein associated with preribosomes in Chinese hamster ovary cells.

Authors:  H M Bourbon; B Bugler; M Caizergues-Ferrer; F Amalric; J P Zalta
Journal:  Mol Biol Rep       Date:  1983-05       Impact factor: 2.316

4.  3'-terminal labelling of RNA with T4 RNA ligase.

Authors:  T E England; O C Uhlenbeck
Journal:  Nature       Date:  1978-10-12       Impact factor: 49.962

5.  A monoclonal antibody against 2,2,7-trimethylguanosine that reacts with intact, class U, small nuclear ribonucleoproteins as well as with 7-methylguanosine-capped RNAs.

Authors:  P Bochnig; R Reuter; P Bringmann; R Lührmann
Journal:  Eur J Biochem       Date:  1987-10-15

6.  Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.

Authors:  E A Lerner; M R Lerner; C A Janeway; J A Steitz
Journal:  Proc Natl Acad Sci U S A       Date:  1981-05       Impact factor: 11.205

7.  The small nucleolar RNP protein NOP1 (fibrillarin) is required for pre-rRNA processing in yeast.

Authors:  D Tollervey; H Lehtonen; M Carmo-Fonseca; E C Hurt
Journal:  EMBO J       Date:  1991-03       Impact factor: 11.598

8.  Purification of snRNPs U1, U2, U4, U5 and U6 with 2,2,7-trimethylguanosine-specific antibody and definition of their constituent proteins reacting with anti-Sm and anti-(U1)RNP antisera.

Authors:  P Bringmann; J Rinke; B Appel; R Reuter; R Lührmann
Journal:  EMBO J       Date:  1983       Impact factor: 11.598

9.  Purification of the individual snRNPs U1, U2, U5 and U4/U6 from HeLa cells and characterization of their protein constituents.

Authors:  P Bringmann; R Lührmann
Journal:  EMBO J       Date:  1986-12-20       Impact factor: 11.598

10.  The yeast homologue of U3 snRNA.

Authors:  J M Hughes; D A Konings; G Cesareni
Journal:  EMBO J       Date:  1987-07       Impact factor: 11.598
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  35 in total

1.  Nuclear retention elements of U3 small nucleolar RNA.

Authors:  W Speckmann; A Narayanan; R Terns; M P Terns
Journal:  Mol Cell Biol       Date:  1999-12       Impact factor: 4.272

Review 2.  Small nucleolar RNAs: versatile trans-acting molecules of ancient evolutionary origin.

Authors:  Michael P Terns; Rebecca M Terns
Journal:  Gene Expr       Date:  2002

3.  An unexpected, conserved element of the U3 snoRNA is required for Mpp10p association.

Authors:  S Wormsley; D A Samarsky; M J Fournier; S J Baserga
Journal:  RNA       Date:  2001-06       Impact factor: 4.942

4.  Interaction of the U3-55k protein with U3 snoRNA is mediated by the box B/C motif of U3 and the WD repeats of U3-55k.

Authors:  A A Lukowiak; S Granneman; S A Mattox; W A Speckmann; K Jones; H Pluk; W J Venrooij; R M Terns; M P Terns
Journal:  Nucleic Acids Res       Date:  2000-09-15       Impact factor: 16.971

5.  Components of U3 snoRNA-containing complexes shuttle between nuclei and the cytoplasm and differentially localize in nucleoli: implications for assembly and function.

Authors:  Daniel J Leary; Michael P Terns; Sui Huang
Journal:  Mol Biol Cell       Date:  2003-10-17       Impact factor: 4.138

6.  Analysis of sequence and structural features that identify the B/C motif of U3 small nucleolar RNA as the recognition site for the Snu13p-Rrp9p protein pair.

Authors:  A Cléry; V Senty-Ségault; F Leclerc; H A Raué; C Branlant
Journal:  Mol Cell Biol       Date:  2006-12-04       Impact factor: 4.272

7.  Mpp10p, a U3 small nucleolar ribonucleoprotein component required for pre-18S rRNA processing in yeast.

Authors:  D A Dunbar; S Wormsley; T M Agentis; S J Baserga
Journal:  Mol Cell Biol       Date:  1997-10       Impact factor: 4.272

8.  Identification of protein binding sites on U3 snoRNA and pre-rRNA by UV cross-linking and high-throughput analysis of cDNAs.

Authors:  Sander Granneman; Grzegorz Kudla; Elisabeth Petfalski; David Tollervey
Journal:  Proc Natl Acad Sci U S A       Date:  2009-05-29       Impact factor: 11.205

9.  Functional mapping of the U3 small nucleolar RNA from the yeast Saccharomyces cerevisiae.

Authors:  D A Samarsky; M J Fournier
Journal:  Mol Cell Biol       Date:  1998-06       Impact factor: 4.272

10.  m3G cap hypermethylation of U1 small nuclear ribonucleoprotein (snRNP) in vitro: evidence that the U1 small nuclear RNA-(guanosine-N2)-methyltransferase is a non-snRNP cytoplasmic protein that requires a binding site on the Sm core domain.

Authors:  G Plessel; U Fischer; R Lührmann
Journal:  Mol Cell Biol       Date:  1994-06       Impact factor: 4.272
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