BACKGROUND: IGF-1 regulates the growth of diverse mammalian cell types including several human carcinoma cell lines. The IGF-1 receptor is a glycosylated heterodimer which, upon binding with IGF-1, undergoes tyrosine autophosphorylation. The autophosphorylation of the beta-receptor subunit is a strict requirement for its mitogenic properties. EXPERIMENTAL DESIGN: In this study, we have assessed the role of the IGF-1 receptor in the proliferation of ovarian carcinoma cell lines in culture. Effects of anti-sense and sense oligodeoxynucleotides to IGF-1 receptor RNA were tested. RESULTS: The human ovarian carcinoma cell lines OVCAR-3 and CaOV-3 both grew autonomously in serum-free medium, and their growth was further stimulated by the addition of IGF-1. Incubation of cells with anti-sense oligodeoxynucleotides corresponding to the IGF-1 receptor RNA markedly inhibited cell proliferation both in serum-free medium and in the presence of IGF-1. The inhibition of cell growth by the oligodeoxynucleotides corresponded to a reduction in the amount of detectable phosphorylated IGF-1 receptor. CONCLUSIONS: The findings indicate that IGF-1 and its specific receptor mediate the autocrine proliferation of human ovarian carcinoma cell lines.
BACKGROUND:IGF-1 regulates the growth of diverse mammalian cell types including several humancarcinoma cell lines. The IGF-1 receptor is a glycosylated heterodimer which, upon binding with IGF-1, undergoes tyrosine autophosphorylation. The autophosphorylation of the beta-receptor subunit is a strict requirement for its mitogenic properties. EXPERIMENTAL DESIGN: In this study, we have assessed the role of the IGF-1 receptor in the proliferation of ovarian carcinoma cell lines in culture. Effects of anti-sense and sense oligodeoxynucleotides to IGF-1 receptor RNA were tested. RESULTS: The humanovarian carcinoma cell lines OVCAR-3 and CaOV-3 both grew autonomously in serum-free medium, and their growth was further stimulated by the addition of IGF-1. Incubation of cells with anti-sense oligodeoxynucleotides corresponding to the IGF-1 receptor RNA markedly inhibited cell proliferation both in serum-free medium and in the presence of IGF-1. The inhibition of cell growth by the oligodeoxynucleotides corresponded to a reduction in the amount of detectable phosphorylated IGF-1 receptor. CONCLUSIONS: The findings indicate that IGF-1 and its specific receptor mediate the autocrine proliferation of humanovarian carcinoma cell lines.
Authors: Albina N Minlikeeva; Kirsten B Moysich; Paul C Mayor; John L Etter; Rikki A Cannioto; Roberta B Ness; Kristen Starbuck; Robert P Edwards; Brahm H Segal; Sashikant Lele; Kunle Odunsi; Brenda Diergaarde; Francesmary Modugno Journal: Cancer Causes Control Date: 2018-01-11 Impact factor: 2.506
Authors: Kathryn L Terry; Shelley S Tworoger; Margaret A Gates; Daniel W Cramer; Susan E Hankinson Journal: Carcinogenesis Date: 2009-12 Impact factor: 4.944