Literature DB >> 82592

A rapid, novel method for the solid-phase derivatization of IgG antibodies for immune-affinity chromatography.

D M Gersten, J J Marchalonis.   

Abstract

We show that a solid-phase immune adsorbent can be prepared from rabbit antiserum by isolating the IgG fraction with the A protein of Staphylococcus aureus associated covalently with a Sepharose matrix. The IgG is then coupled to the matrix using the cross-linking agent dimethylsuberimidate. IgG antibody bound in this fashion is in the proper orientation for combination with antigen because association with protein A occurs via the Fc portion of the IgG molecule, thus leaving the combining site of the molecule free to interact with antigen.

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Year:  1978        PMID： 82592     DOI： 10.1016/0022-1759(78)90133-3

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  26 in total

1.  Identification of sulfated oligosialic acid units in the O-linked glycan of the sea urchin egg receptor for sperm.

Authors:  S Kitazume-Kawaguchi; S Inoue; Y Inoue; W J Lennarz
Journal:  Proc Natl Acad Sci U S A       Date:  1997-04-15       Impact factor: 11.205

2.  Activation of phospholipase D is an early event in integrin-mediated signalling leading to phagocytosis in human neutrophils.

Authors:  L Serrander; M Fällman; O Stendahl
Journal:  Inflammation       Date:  1996-08       Impact factor: 4.092

3.  Pre-mRNA splicing by complementation with purified human U1, U2, U4/U6 and U5 snRNPs.

Authors:  A R Krainer
Journal:  Nucleic Acids Res       Date:  1988-10-25       Impact factor: 16.971

4.  Direct cross-linking of snRNP proteins F and 70K to snRNAs by ultra-violet radiation in situ.

Authors:  A Woppmann; J Rinke; R Lührmann
Journal:  Nucleic Acids Res       Date:  1988-12-09       Impact factor: 16.971

5.  Characterization of immunologically significant unique B16 melanoma proteins produced in vivo and in vitro.

Authors:  D M Gersten; V J Hearing; J J Marchalonis
Journal:  Proc Natl Acad Sci U S A       Date:  1981-08       Impact factor: 11.205

6.  Purification by Immunoadsorption and Immunochemical Properties of NADP-Dependent Malic Enzymes from Leaves of C(3), C(4), and Crassulacean Acid Metabolism Plants.

Authors:  M Fathi; C Schnarrenberger
Journal:  Plant Physiol       Date:  1990-03       Impact factor: 8.340

7.  Oriented covalent immobilization of antibodies for measurement of intermolecular binding forces between zipper-like contact surfaces of split inteins.

Authors:  Mirco Sorci; Bareket Dassa; Hongwei Liu; Gaurav Anand; Amit K Dutta; Shmuel Pietrokovski; Marlene Belfort; Georges Belfort
Journal:  Anal Chem       Date:  2013-05-31       Impact factor: 6.986

8.  Monoclonal antibody recognizing gp80, a membrane glycoprotein implicated in intercellular adhesion of Dictyostelium discoideum.

Authors:  B A Murray; H L Niman; W F Loomis
Journal:  Mol Cell Biol       Date:  1983-05       Impact factor: 4.272

9.  Linkage of 17 beta-oestradiol dehydrogenase to actin by epsilon-(gamma-glutamyl)-lysine in porcine endometrial cells.

Authors:  J Adamski; B Husen; H H Thole; U Groeschel-Stewart; P W Jungblut
Journal:  Biochem J       Date:  1993-12-15       Impact factor: 3.857

10.  Temperature tolerance of hydrogenase expression in Alcaligenes eutrophus is conferred by a single amino acid exchange in the transcriptional activator HoxA.

Authors:  D Zimmer; E Schwartz; A Tran-Betcke; P Gewinner; B Friedrich
Journal:  J Bacteriol       Date:  1995-05       Impact factor: 3.490
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