Literature DB >> 8258297

Characterization of a human chromosome 4 flow-sorted cosmid library.

O Riess1, I Siedlaczck, S Kredtke, G Melmer, J T Epplen, L L Deaven.   

Abstract

A cosmid library has been constructed from a hamster x human hybrid cell line and gridded into 270 microtiter plates containing a total of 25,920 single colonies. Approximately 84% of the recombinants contain human material, with an average length of 29 kb. This library represents a nearly three-fold coverage of human chromosome 4. We investigated this library for presumptive genes, using a set of oligonucleotides detecting SpI and splice-site consensus sequences. The presence of simple repeat motifs was investigated in the cosmids using the oligonucleotides (GGATTT)3, (GGAT)4, (CAC)5, (GCC)5, (AGC)5, (GATA)4, (GACA)4, and (CA)8 as hybridization probes.

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Year:  1994        PMID： 8258297     DOI： 10.1159/000133638

Source DB:  PubMed          Journal:  Cytogenet Cell Genet        ISSN： 0301-0171


  4 in total

1.  Construction of chromosome-specific DNA libraries covering the whole genome of field bean (Vicia faba L.).

Authors:  J Macas; G Gualberti; M Nouzová; P Samec; S Lucretti; J Dolezel
Journal:  Chromosome Res       Date:  1996-11       Impact factor: 5.239

Review 2.  Toward the complete genomic map and molecular pathology of human chromosome 4.

Authors:  O Riess; B Winkelmann; J T Epplen
Journal:  Hum Genet       Date:  1994-07       Impact factor: 4.132

3.  Analysis of allele-specific RNA transcription in FSHD by RNA-DNA FISH in single myonuclei.

Authors:  Peter S Masny; On Ying A Chan; Jessica C de Greef; Ulla Bengtsson; Melanie Ehrlich; Rabi Tawil; Leslie F Lock; Jane E Hewitt; Jennifer Stocksdale; Jorge H Martin; Silvere M van der Maarel; Sara T Winokur
Journal:  Eur J Hum Genet       Date:  2009-11-04       Impact factor: 4.246

4.  Mechanisms of small ring formation suggested by the molecular characterization of two small accessory ring chromosomes derived from chromosome 4.

Authors:  Y Y Fang; H J Eyre; S K Bohlander; A Estop; E McPherson; T Träger; O Riess; D F Callen
Journal:  Am J Hum Genet       Date:  1995-11       Impact factor: 11.025
  4 in total

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