Increased generation of superoxide radicals in the blood of MS patients.

Increased generation of superoxide radical (O2-) in the blood stimulated with phorbol myristate acetate (PMA) has been found in multiple sclerosis (MS) patients. All the subjects studied were non-smokers and received the same hospital diet. In the MS group treated with prednisone superoxide radical generation was lower than in untreated patients, but was still above the level of O2- generation in controls. Plasma lipid peroxidation products, measured as thiobarbituric-reactive substances, were slightly elevated in both prednisone treated and non-treated MS patients in comparison with controls. Erythrocyte superoxide dismutase activity (SOD) was at the same level in MS and control groups. These results suggest that increased generation of reactive oxygen species may occur in MS patients during acute relapse. This phenomenon may be involved in the pathomechanism of demyelinating disorders.