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Literature DB >> 8254773

Screening retroviral packaging cells for highly efficient virus production by using a combined selection procedure.

M Wirth¹, R Grannemann, D Klehr, H Hauser.

Abstract

To facilitate the screening for clones of transfected packaging cells producing a high yield of recombinant retrovirus, we present a fast and simple method for the isolation of overexpressing cells. By this method the efficiency of virus production can generally be enhanced 10- to 100-fold by application of high selection pressure. Cell lines which exhibit titers of up to 10(8) CFU/ml were obtained.

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Year: 1994 PMID： 8254773 PMCID： PMC236323

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

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References

19 in total

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Authors: A D Miller; G J Rosman
Journal: Biotechniques Date: 1989-10 Impact factor: 1.993

2. Characterization of recombinant helper retroviruses from Moloney-based vectors in ecotropic and amphotropic packaging cell lines.

Authors: M Scarpa; D Cournoyer; D M Muzny; K A Moore; J W Belmont; C T Caskey
Journal: Virology Date: 1991-02 Impact factor: 3.616

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Authors: J P Morgenstern; H Land
Journal: Nucleic Acids Res Date: 1990-06-25 Impact factor: 16.971

4. Isolation of overproducing recombinant mammalian cell lines by a fast and simple selection procedure.

Authors: M Wirth; J Bode; G Zettlmeissl; H Hauser
Journal: Gene Date: 1988-12-20 Impact factor: 3.688

5. Development of a high-titer retrovirus producer cell line capable of gene transfer into rhesus monkey hematopoietic stem cells.

Authors: D M Bodine; K T McDonagh; S J Brandt; P A Ney; B Agricola; E Byrne; A W Nienhuis
Journal: Proc Natl Acad Sci U S A Date: 1990-05 Impact factor: 11.205

Screening retroviral packaging cells for highly efficient virus production by using a combined selection procedure.

1. Improved retroviral vectors for gene transfer and expression.

2. Characterization of recombinant helper retroviruses from Moloney-based vectors in ecotropic and amphotropic packaging cell lines.

3. Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line.

4. Isolation of overproducing recombinant mammalian cell lines by a fast and simple selection procedure.

5. Development of a high-titer retrovirus producer cell line capable of gene transfer into rhesus monkey hematopoietic stem cells.

6. Ping-pong amplification of a retroviral vector achieves high-level gene expression: human growth hormone production.

7. Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus.

8. Plaque assay techniques for murine leukemia viruses.

9. Construction and properties of replication-competent murine retroviral vectors encoding methotrexate resistance.

10. New retrovirus helper cells with almost no nucleotide sequence homology to retrovirus vectors.