Literature DB >> 8253985

Simple and rapid two-step polymerase chain reaction for diagnosis of Pneumocystis carinii infection.

E Tamburrini1, P Mencarini, A De Luca, A Antinori, E Visconti, A Ammassari, L Ortona, E Ortona, A Siracusano, G Vicari.   

Abstract

Two amplification steps were made to detect Pneumocystis carinii DNA by polymerase chain reaction (PCR). pAZ102-E and pAZ102-H (standard PCR), pAZ102-L2 (sense), and pAZ102-E (antisense) (two-step PCR) were used as primers. The amplification products were analyzed by ethidium bromide. After the two-step PCR, ethidium bromide detected all samples positive by oligohybridization after one amplification step. Our two-step PCR is a rapid, cost-effective, and clinically suitable method for the detection of P. carinii infection.

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Year:  1993        PMID： 8253985      PMCID： PMC266016          DOI： 10.1128/jcm.31.10.2788-2789.1993

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  5 in total

1.  Improved diagnosis of Pneumocystis carinii infection by polymerase chain reaction on induced sputum and blood.

Authors:  G Y Lipschik; V J Gill; J D Lundgren; V A Andrawis; N A Nelson; J O Nielsen; F P Ognibene; J A Kovacs
Journal:  Lancet       Date:  1992-07-25       Impact factor: 79.321

2.  Detection of Pneumocystis carinii DNA in sputum and bronchoalveolar lavage samples by polymerase chain reaction.

Authors:  M Olsson; K Elvin; S Löfdahl; E Linder
Journal:  J Clin Microbiol       Date:  1993-02       Impact factor: 5.948

3.  Correlation of morphologic diagnosis of Pneumocystis carinii with the presence of pneumocystis DNA amplified by the polymerase chain reaction.

Authors:  W Blumenfeld; O McCook; M Holodniy; D A Katzenstein
Journal:  Mod Pathol       Date:  1992-03       Impact factor: 7.842

4.  Detection of Pneumocystis carinii with DNA amplification.

Authors:  A E Wakefield; F J Pixley; S Banerji; K Sinclair; R F Miller; E R Moxon; J M Hopkin
Journal:  Lancet       Date:  1990-08-25       Impact factor: 79.321

5.  Diagnosis of Pneumocystis carinii pneumonia: specificity and sensitivity of polymerase chain reaction in comparison with immunofluorescence in bronchoalveolar lavage specimens.

Authors:  E Tamburrini; P Mencarini; A De Luca; G Maiuro; G Ventura; A Antinori; A Ammassari; E Visconti; L Ortona; A Siracusano
Journal:  J Med Microbiol       Date:  1993-06       Impact factor: 2.472
  5 in total
  4 in total

1.  Evaluation of diagnostic value and epidemiological implications of PCR for Pneumocystis carinii in different immunosuppressed and immunocompetent patient groups.

Authors:  A Sing; K Trebesius; A Roggenkamp; H Rüssmann; K Tybus; F Pfaff; J R Bogner; C Emminger; J Heesemann
Journal:  J Clin Microbiol       Date:  2000-04       Impact factor: 5.948

2.  Usefulness of PCR for diagnosis of Pneumocystis carinii pneumonia in different patient groups.

Authors:  M Weig; H Klinker; B H Bögner; A Meier; U Gross
Journal:  J Clin Microbiol       Date:  1997-06       Impact factor: 5.948

3.  Detection of Pneumocystis carinii in respiratory specimens by PCR-solution hybridization enzyme-linked immunoassay.

Authors:  E Ortona; P Margutti; E Tamburrini; P Mencarini; E Visconti; M Zolfo; A Siracusano
Journal:  J Clin Microbiol       Date:  1997-06       Impact factor: 5.948

4.  Rapid detection of Pneumocystis carinii in bronchoalveolar lavage specimens from human immunodeficiency virus-infected patients: use of a simple DNA extraction procedure and nested PCR.

Authors:  M Rabodonirina; D Raffenot; L Cotte; A Boibieux; M Mayençon; G Bayle; F Persat; F Rabatel; C Trepo; D Peyramond; M A Piens
Journal:  J Clin Microbiol       Date:  1997-11       Impact factor: 5.948
  4 in total

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