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Literature DB >> 8253980

Differentiation of strains of Actinobacillus actinomycetemcomitans by arbitrarily primed polymerase chain reaction.

H R Preus¹, V I Haraszthy, J J Zambon, R J Genco.

Abstract

The present study describes a method for amplifying DNA in Actinobacillus actinomycetemcomitans by using short, synthetic oligonucleotides of random sequence as primers in the polymerase chain reaction. Genomic DNA from each of 20 human isolates of A. actinomycetemcomitans was successfully amplified in a thermal cycler with a single synthetic primer (GGGTAACGCC) and reproducibly produced 14 different DNA amplification profiles (amplitypes). A. actinomycetemcomitans isolates from the same subject revealed the same amplitype. The arbitrarily primed polymerase chain reaction appears to be useful in characterizing human isolates of A. actinomycetemcomitans for studies of epidemiology and bacterial transmission.

Entities: Chemical Species

Mesh：

Substances：
DNA, Bacterial

Year: 1993 PMID： 8253980 PMCID： PMC266011 DOI： 10.1128/jcm.31.10.2773-2776.1993

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

11 in total

1. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.

Authors: J G Williams; A R Kubelik; K J Livak; J A Rafalski; S V Tingey
Journal: Nucleic Acids Res Date: 1990-11-25 Impact factor: 16.971

2. Possible transmittance of A. actinomycetemcomitans from a dog to a child with rapidly destructive periodontitis.

Authors: H R Preus; I Olsen
Journal: J Periodontal Res Date: 1988-01 Impact factor: 4.419

3. Fingerprinting genomes using PCR with arbitrary primers.

Authors: J Welsh; M McClelland
Journal: Nucleic Acids Res Date: 1990-12-25 Impact factor: 16.971

4. Molecular genetic analysis of Actinobacillus actinomycetemcomitans epidemiology.

Authors: J J Zambon; G J Sunday; J S Smutko
Journal: J Periodontol Date: 1990-02 Impact factor: 6.993

5. Selective medium for isolation of Actinobacillus actinomycetemcomitans.

Authors: J Slots
Journal: J Clin Microbiol Date: 1982-04 Impact factor: 5.948

6. Rapid identification of Actinobacillus actinomycetemcomitans based on analysis of 23S ribosomal RNA.

Authors: H R Preus; G J Sunday; V I Haraszthy; J J Zambon
Journal: Oral Microbiol Immunol Date: 1992-12

7. Actinobacillus actinomycetemcomitans in human periodontal disease: a cross-sectional microbiological investigation.

Authors: J Slots; H S Reynolds; R J Genco
Journal: Infect Immun Date: 1980-09 Impact factor: 3.441

8. Genetic approach to the study of epidemiology and pathogenesis of Actinobacillus actinomycetemcomitans in localized juvenile periodontitis.

Authors: J M DiRienzo; J Slots
Journal: Arch Oral Biol Date: 1990 Impact factor: 2.633

Differentiation of strains of Actinobacillus actinomycetemcomitans by arbitrarily primed polymerase chain reaction.

1. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.

2. Possible transmittance of A. actinomycetemcomitans from a dog to a child with rapidly destructive periodontitis.

3. Fingerprinting genomes using PCR with arbitrary primers.

4. Molecular genetic analysis of Actinobacillus actinomycetemcomitans epidemiology.

5. Selective medium for isolation of Actinobacillus actinomycetemcomitans.

6. Rapid identification of Actinobacillus actinomycetemcomitans based on analysis of 23S ribosomal RNA.

7. Actinobacillus actinomycetemcomitans in human periodontal disease: a cross-sectional microbiological investigation.

8. Genetic approach to the study of epidemiology and pathogenesis of Actinobacillus actinomycetemcomitans in localized juvenile periodontitis.

Review 9. Actinobacillus actinomycetemcomitans in human periodontal disease.

10. Serology of oral Actinobacillus actinomycetemcomitans and serotype distribution in human periodontal disease.

1. Identification of Actinobacillus actinomycetemcomitans serotypes by multiplex PCR.

2. Characterization of serologically nontypeable Actinobacillus actinomycetemcomitans isolates.

3. Epidemiological typing of Stenotrophomonas (Xanthomonas) maltophilia by PCR.