Literature DB >> 8251640

Cloning, sequence analysis and expression of a cDNA encoding active phosphoenolpyruvate carboxylase of the C3 plant Solanum tuberosum.

S Merkelbach1, J Gehlen, M Denecke, H J Hirsch, F Kreuzaler.   

Abstract

A cDNA coding for phosphoenolpyruvate carboxylase (PEPC) was isolated from a cDNA library from Solanum tuberosum and the sequence of the cDNA was determined. It was inserted into a bacterial expression vector and a PEPC- Escherichia coli mutant could be complemented by the cDNA construct. A functional fusion protein could be synthesized in E. coli. The properties of this PEPC protein clearly resembled those of typical C3 plant enzymes.

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Year:  1993        PMID: 8251640     DOI: 10.1007/bf00021542

Source DB:  PubMed          Journal:  Plant Mol Biol        ISSN: 0167-4412            Impact factor:   4.076


  17 in total

1.  Multiple cDNAs of phosphoenolpyruvate carboxylase in the C4 dicot Flaveria trinervia.

Authors:  W Poetsch; J Hermans; P Westhoff
Journal:  FEBS Lett       Date:  1991-11-04       Impact factor: 4.124

2.  Sequence analysis of cDNA encoding phosphoenolpyruvate carboxylase from cultured tobacco cells.

Authors:  N Koizumi; F Sato; Y Terano; Y Yamada
Journal:  Plant Mol Biol       Date:  1991-09       Impact factor: 4.076

3.  Expression of the CAM-form of phospho(enol)pyruvate carboxylase and nucleotide sequence of a full length cDNA from Mesembryanthemum crystallinum.

Authors:  J Rickers; J C Cushman; C B Michalowski; J M Schmitt; H J Bohnert
Journal:  Mol Gen Genet       Date:  1989-02

4.  The reduction and restoration of galactose transport in osmotically shocked cells of Escherichia coli.

Authors:  Y Anraku
Journal:  J Biol Chem       Date:  1967-03-10       Impact factor: 5.157

5.  Cloning and sequence analysis of cDNA encoding active phosphoenolpyruvate carboxylase of the C4-pathway from maize.

Authors:  K Izui; S Ishijima; Y Yamaguchi; F Katagiri; T Murata; K Shigesada; T Sugiyama; H Katsuki
Journal:  Nucleic Acids Res       Date:  1986-02-25       Impact factor: 16.971

6.  In vivo regulatory phosphorylation site in c(4)-leaf phosphoenolpyruvate carboxylase from maize and sorghum.

Authors:  J A Jiao; J Vidal; C Echevarría; R Chollet
Journal:  Plant Physiol       Date:  1991-05       Impact factor: 8.340

7.  cDNA sequence and expression of a phosphoenolpyruvate carboxylase gene from soybean.

Authors:  T Sugimoto; T Kawasaki; T Kato; R F Whittier; D Shibata; Y Kawamura
Journal:  Plant Mol Biol       Date:  1992-11       Impact factor: 4.076

8.  Regulatory phosphorylation of Sorghum leaf phosphoenolpyruvate carboxylase. Identification of the protein-serine kinase and some elements of the signal-transduction cascade.

Authors:  N Bakrim; C Echevarria; C Cretin; M Arrio-Dupont; J N Pierre; J Vidal; R Chollet; P Gadal
Journal:  Eur J Biochem       Date:  1992-03-01

9.  Homologous genes for the C4 isoform of phosphoenolpyruvate carboxylase in a C3 and a C4 Flaveria species.

Authors:  J Hermans; P Westhoff
Journal:  Mol Gen Genet       Date:  1992-08

10.  Molecular cloning of the phosphoenolpyruvate carboxylase gene, ppc, of Escherichia coli.

Authors:  H Sabe; T Miwa; T Kodaki; K Izui; S Hiraga; H Katsuki
Journal:  Gene       Date:  1984-11       Impact factor: 3.688

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  3 in total

1.  Purification and characterization of high- and low-molecular-mass isoforms of phosphoenolpyruvate carboxylase from Chlamydomonas reinhardtii. Kinetic, structural and immunological evidence that the green algal enzyme is distinct from the prokaryotic and higher plant enzymes.

Authors:  J Rivoal; W C Plaxton; D H Turpin
Journal:  Biochem J       Date:  1998-04-01       Impact factor: 3.857

2.  Molecular characterization of a phosphoenolpyruvate carboxylase in the gymnosperm Picea abies (Norway spruce).

Authors:  M Relle; A Wild
Journal:  Plant Mol Biol       Date:  1996-12       Impact factor: 4.076

3.  Effects of altered phosphoenolpyruvate carboxylase activities on transgenic C3 plant Solanum tuberosum.

Authors:  J Gehlen; R Panstruga; H Smets; S Merkelbach; M Kleines; P Porsch; M Fladung; I Becker; T Rademacher; R E Häusler; H J Hirsch
Journal:  Plant Mol Biol       Date:  1996-12       Impact factor: 4.076

  3 in total

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