Literature DB >> 8251167

Nonradioactive gel mobility shift assay using chemiluminescent detection.

R Berger1, M R Duncan, B Berman.   

Abstract

A nonradioactive gel mobility shift assay using chemiluminescent detection following semidry transfer to nylon membranes is described. The procedure utilizes digoxigenin-labeled oligonucleotides in conjunction with anti-digoxigenin antibody Fab fragments coupled to alkaline phosphatase. Detection of alkaline phosphatase is by autoradiography of the chemiluminescence produced during enzymatic dephosphorylation of a dioxetane substrate. This method offers similar sensitivity to radioactive methods while having the advantages of multiple exposures, faster processing, stability of labeled oligonucleotides and safety associated with nonradioactive methods of detection.

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Year:  1993        PMID： 8251167

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  7 in total

1.  Characterization of heparin-living bacteria interactions by chemiluminescence electrophoretic mobility shift assay.

Authors:  Jonghoon Kang; Myung Soog Lee; David G Gorenstein
Journal:  Anal Biochem       Date:  2007-02-02       Impact factor: 3.365

2.  Electrophoretic mobility shift assay (EMSA) for detecting protein-nucleic acid interactions.

Authors:  Lance M Hellman; Michael G Fried
Journal:  Nat Protoc       Date:  2007       Impact factor: 13.491

3.  Using FAM labeled DNA oligos to do RNA electrophoretic mobility shift assay.

Authors:  Kun Wang; Ya Gao; Xiaojue Peng; Guohua Yang; Feng Gao; Shaoqing Li; Yingguo Zhu
Journal:  Mol Biol Rep       Date:  2009-09-26       Impact factor: 2.316

4.  Gel-Based Analysis of Protein-Nucleic Acid Interactions.

Authors:  James A W Stowell; Terence T L Tang; Maximilian Seidel; Lori A Passmore
Journal:  Methods Mol Biol       Date:  2021

5.  Norcantharidin-induced apoptosis is via the extracellular signal-regulated kinase and c-Jun-NH2-terminal kinase signaling pathways in human hepatoma HepG2 cells.

Authors:  Yan-Nian Chen; Chi-Chih Cheng; Jung-Chou Chen; Wei Tsauer; Shih-Lan Hsu
Journal:  Br J Pharmacol       Date:  2003-09-01       Impact factor: 8.739

6.  Analyzing the nuclear complexes of Notch signaling by electrophoretic mobility shift assay.

Authors:  Kelly L Arnett; Stephen C Blacklow
Journal:  Methods Mol Biol       Date:  2014

7.  Inhibition of TNFalpha-induced cyclooxygenase-2 expression by amentoflavone through suppression of NF-kappaB activation in A549 cells.

Authors:  Tinku Banerjee; Giuseppe Valacchi; Vincent A Ziboh; Albert van der Vliet
Journal:  Mol Cell Biochem       Date:  2002-09       Impact factor: 3.396
  7 in total

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