Nucleolar accumulation of core protein in cells naturally infected with Semliki Forest virus. Quantitative aspects.

The core (C) protein of Semliki Forest virus (SFV) is known to exert several important functions with regard to both the virus and the host. This paper shows that migration of parental and progeny C protein to the nucleolus is a common feature in infected vertebrate and invertebrate cells. The amount of C protein accumulating to the nucleolus is small, always less than 1% of the intra- and extracellular C protein at various times post infection. Migration to the nucleolus is a fast process; 1.5 h post infection a prominent amount of parental C protein is already incorporated into nucleolar fractions.