Literature DB >> 8248103

Production and properties of a factor X-cellulose-binding domain fusion protein.

Z Assouline1, H Shen, D G Kilburn, R A Warren.   

Abstract

A fusion protein, FX-CBDCex, which comprises factor X with a cellulose-binding domain (CBDCex) fused to its C-terminus, was produced in BHK cells. It was purified from the culture medium by affinity chromatography on cellulose. FX-CBDCex could be activated to FXa-CBDCex with Russell viper venom. FXa-CBDCex was as active as FXa against a chromogenic substrate and against proteins containing the Ile-Glu-Gly-Arg sequence hydrolysed by FXa. FXa-CBDCex retained its activity when adsorbed to cellulose.

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Year:  1993        PMID： 8248103     DOI： 10.1093/protein/6.7.787

Source DB:  PubMed          Journal:  Protein Eng        ISSN： 0269-2139


  3 in total

1.  Specific adhesion to cellulose and hydrolysis of organophosphate nerve agents by a genetically engineered Escherichia coli strain with a surface-expressed cellulose-binding domain and organophosphorus hydrolase.

Authors:  Aijun A Wang; Ashok Mulchandani; Wilfred Chen
Journal:  Appl Environ Microbiol       Date:  2002-04       Impact factor: 4.792

2.  Production of a self-activating CBM-factor X fusion protein in a stable transformed Sf9 insect cell line using high cell density perfusion culture.

Authors:  Volker M Gorenflo; Tom A Pfeifer; Gary Lesnicki; Emily M Kwan; Thomas A Grigliatti; Douglas G Kilburn; James M Piret
Journal:  Cytotechnology       Date:  2004-03       Impact factor: 2.058

3.  Cellulose as an inert matrix for presenting cytokines to target cells: production and properties of a stem cell factor-cellulose-binding domain fusion protein.

Authors:  J G Doheny; E J Jervis; M M Guarna; R K Humphries; R A Warren; D G Kilburn
Journal:  Biochem J       Date:  1999-04-15       Impact factor: 3.857
  3 in total

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