Literature DB >> 8245425

Fundamentals of quantitative autoradiography by computer densitometry for in situ hybridization, with emphasis on 33P.

D G Baskin1, W L Stahl.   

Abstract

The goal of quantitative autoradiography (QAR) in in situ hybridization (ISH) is to determine the amount of radioactive oligonucleotide or riboprobe present in the corresponding area of the tissue slice that produced an autoradiographic image. This article discusses (a) some of the considerations related to selection and use of computer image analysis systems for accomplishing this objective, (b) development of 14C plastic autoradiographic standards for ISH QAR with 33P, (c) using QAR to develop Tm curves for ISH probes, and (d) measurement of resolution with video imaging systems for QAR.

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Year:  1993        PMID： 8245425     DOI： 10.1177/41.12.8245425

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN： 0022-1554            Impact factor:   2.479


  9 in total

1.  Autoradiographic localization of the putative D4 dopamine receptor in rat brain.

Authors:  M C Defagot; M C Antonelli
Journal:  Neurochem Res       Date:  1997-04       Impact factor: 3.996

2.  Pathological expression of renin and angiotensin II in the renal tubule after subtotal nephrectomy. Implications for the pathogenesis of tubulointerstitial fibrosis.

Authors:  R E Gilbert; L L Wu; D J Kelly; A Cox; J L Wilkinson-Berka; C I Johnston; M E Cooper
Journal:  Am J Pathol       Date:  1999-08       Impact factor: 4.307

3.  Analysis of messenger RNA expression by in situ hybridization using RNA probes synthesized via in vitro transcription.

Authors:  Bradley S Carter; Jonathan S Fletcher; Robert C Thompson
Journal:  Methods       Date:  2010-08-10       Impact factor: 3.608

4.  Synthetic Oxytocin and Vasopressin Act Within the Central Amygdala to Exacerbate Aggression in Female Wistar Rats.

Authors:  Vinícius E de M Oliveira; Trynke R de Jong; Inga D Neumann
Journal:  Front Neurosci       Date:  2022-05-18       Impact factor: 5.152

5.  Expression, localization, and function of the thioredoxin system in diabetic nephropathy.

Authors:  Andrew Advani; Richard E Gilbert; Kerri Thai; Renae M Gow; Robyn G Langham; Alison J Cox; Kim A Connelly; Yuan Zhang; Andrew M Herzenberg; Per Knud Christensen; Carol A Pollock; Weier Qi; Sih Min Tan; Hans-Henrik Parving; Darren J Kelly
Journal:  J Am Soc Nephrol       Date:  2009-02-11       Impact factor: 10.121

6.  Oxytocin and vasopressin within the ventral and dorsal lateral septum modulate aggression in female rats.

Authors:  Vinícius Elias de Moura Oliveira; Michael Lukas; Hannah Nora Wolf; Elisa Durante; Alexandra Lorenz; Anna-Lena Mayer; Anna Bludau; Oliver J Bosch; Valery Grinevich; Veronica Egger; Trynke R de Jong; Inga D Neumann
Journal:  Nat Commun       Date:  2021-05-18       Impact factor: 14.919

7.  Statistical evaluation of methods for quantifying gene expression by autoradiography in histological sections.

Authors:  Stanley E Lazic
Journal:  BMC Neurosci       Date:  2009-01-15       Impact factor: 3.288

8.  Role of VEGF in maintaining renal structure and function under normotensive and hypertensive conditions.

Authors:  Andrew Advani; Darren J Kelly; Suzanne L Advani; Alison J Cox; Kerri Thai; Yuan Zhang; Kathryn E White; Renae M Gow; Sally M Marshall; Brent M Steer; Philip A Marsden; P Elizabeth Rakoczy; Richard E Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  2007-08-28       Impact factor: 11.205

9.  Quantitative methods for genome-scale analysis of in situ hybridization and correlation with microarray data.

Authors:  Chang-Kyu Lee; Susan M Sunkin; Chihchau Kuan; Carol L Thompson; Sayan Pathak; Lydia Ng; Chris Lau; Shanna Fischer; Marty Mortrud; Cliff Slaughterbeck; Allan Jones; Ed Lein; Michael Hawrylycz
Journal:  Genome Biol       Date:  2008-01-30       Impact factor: 13.583
  9 in total

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