Literature DB >> 8245421

A method for simultaneous detection of multiple mRNAs using digoxigenin and radioisotopic cRNA probes.

M A Miller1, P E Kolb, M A Raskind.   

Abstract

We present a simple, reliable method for simultaneous detection of two distinct mRNAs within the same tissue sample by double in situ hybridization histochemistry. Sections are hybridized with a cocktail of radiolabeled and digoxigenin-labeled cRNA probes. The digoxigenin-labeled probe is detected with an alkaline phosphatase (AP)-dependent chromogen reaction and then the radiolabeled probe is detected by conventional autoradiography. The sensitivities of the two detection methods are comparable and demonstrate the feasibility of using double in situ hybridization histochemistry to investigate the co-localization and co-regulation of mRNAs that are expressed at relatively low levels.

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Year:  1993        PMID： 8245421     DOI： 10.1177/41.12.8245421

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN： 0022-1554            Impact factor:   2.479


  6 in total

1.  The LIM-homeodomain protein Isl-1 segregates with somatostatin but not with gastrin expression during differentiation of somatostatin/gastrin precursor cells.

Authors:  L I Larsson; J E Tingstedt; O D Madsen; P Serup; D M Hougaard
Journal:  Endocrine       Date:  1995-07       Impact factor: 3.633

Review 2.  In situ hybridization: methods and applications.

Authors:  L Jin; R V Lloyd
Journal:  J Clin Lab Anal       Date:  1997       Impact factor: 2.352

3.  Detection of ebaf, a novel human gene of the transforming growth factor beta superfamily association of gene expression with endometrial bleeding.

Authors:  R Kothapalli; I Buyuksal; S Q Wu; N Chegini; S Tabibzadeh
Journal:  J Clin Invest       Date:  1997-05-15       Impact factor: 14.808

4.  Expression of type I and IV collagen mRNAs in healing gastric ulcers--a comparative analysis using isotopic and non-radioactive in situ hybridization.

Authors:  T Pohle; M Shahin; A Gillessen; D Schuppan; H Herbst; W Domschke
Journal:  Histochem Cell Biol       Date:  1996-10       Impact factor: 4.304

5.  Signal intensities of radiolabeled cRNA probes used alone or in combination with non-isotopic in situ hybridization histochemistry.

Authors:  Jong-Hyun Son; Ursula H Winzer-Serhan
Journal:  J Neurosci Methods       Date:  2009-02-04       Impact factor: 2.390

6.  Single Molecule Analysis of Laser Localized Interstrand Crosslinks.

Authors:  Jing Huang; Himabindu Gali; Manikandan Paramasivam; Parameswary Muniandy; Julia Gichimu; Marina A Bellani; Michael M Seidman
Journal:  Front Genet       Date:  2016-05-09       Impact factor: 4.599
  6 in total

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