Literature DB >> 8243981

Use of RAPD-PCR to isolate a species specific DNA probe for Phytophthora cinnamomi.

M P Dobrowolski1, P A O'Brien.   

Abstract

The products of RAPD-PCR amplification of Phytophthora cinnamomi DNA were separated by electrophoresis in agarose. Parallel Southern blots of the gels were hybridized with nick translated DNA from different species of Phytophthora. Fragments that hybridized specifically to P. cinnamomi DNA were identified. These fragments were purified and cloned into pUC18. Their specificity for P. cinnamomi was confirmed.

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Year:  1993        PMID: 8243981     DOI: 10.1111/j.1574-6968.1993.tb06485.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN: 0378-1097            Impact factor:   2.742


  1 in total

1.  Development of SCAR Markers for the Identification of Phytophthora katsurae Causing Chestnut Ink Disease in Korea.

Authors:  Dong Hyeon Lee; Sun Keun Lee; Sang Yong Lee; Jong Kyu Lee
Journal:  Mycobiology       Date:  2013-06-30       Impact factor: 1.858

  1 in total

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