Literature DB >> 8237465

Glial fibrillary acidic protein and its encoding mRNA exhibit mosaic expression in a glioblastoma multiform cell line of clonal origin.

V Bocchini1, T Beccari, C Arcuri, L Bruyere, C Fages, M Tardy.   

Abstract

The expression of two astroglial differentiation markers, vimentin and glial fibrillary acidic protein, was investigated in a previously established human glioma cell line of clonal origin (GL15). Vimentin immunolabelling was homogeneously expressed in all cells. Glial fibrillary acidic protein and its encoding message, investigated by immunocytochemistry and in situ hybridization, showed a mosaic-like expression. Only 30% of the cell population expressed glial fibrillary acidic protein and its mRNA. Western and Northern blots performed for both markers confirmed the presence of both proteins and messages, and their level was correlated with the observed antigenic and molecular probe labelling. The overall antigenic pattern suggests that GL-15 cells do not belong to the O-2A progenitor cell lineage and may arise from a clonal expansion of astrocyte precursors.

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Year:  1993        PMID： 8237465     DOI： 10.1016/0736-5748(93)90022-6

Source DB:  PubMed          Journal:  Int J Dev Neurosci        ISSN： 0736-5748            Impact factor:   2.457


  6 in total

1.  Glutamine synthetase gene expression in a glioblastoma cell-line of clonal origin: regulation by dexamethasone and dibutyryl cyclic AMP.

Authors:  C Arcuri; M Tardy; B Rolland; R Armellini; A R Menghini; V Bocchini
Journal:  Neurochem Res       Date:  1995-10       Impact factor: 3.996

2.  Migration pathways of human glioblastoma cells xenografted into the immunosuppressed rat brain.

Authors:  J S Guillamo; F Lisovoski; C Christov; C Le Guérinel; G L Defer; M Peschanski; T Lefrançois
Journal:  J Neurooncol       Date:  2001-05       Impact factor: 4.130

3.  A time-dependent increase in glial fibrillary acidic protein expression and glutamine synthetase activity in long-term subculture of the GL15 glioma cell line.

Authors:  G Moretto; N Brutti; V De Angelis; C Arcuri; V Bocchini
Journal:  Cell Mol Neurobiol       Date:  1997-10       Impact factor: 5.046

4.  Multidrug resistance-associated protein MRP1 expression in human gliomas: chemosensitization to vincristine and etoposide by indomethacin in human glioma cell lines overexpressing MRP1.

Authors:  B Benyahia; S Huguet; X Declèves; K Mokhtari; E Crinière; J F Bernaudin; J M Scherrmann; J Y Delattre
Journal:  J Neurooncol       Date:  2004-01       Impact factor: 4.130

5.  RCCS bioreactor-based modelled microgravity induces significant changes on in vitro 3D neuroglial cell cultures.

Authors:  Caterina Morabito; Nathalie Steimberg; Giovanna Mazzoleni; Simone Guarnieri; Giorgio Fanò-Illic; Maria A Mariggiò
Journal:  Biomed Res Int       Date:  2015-01-13       Impact factor: 3.411

6.  PP242 Counteracts Glioblastoma Cell Proliferation, Migration, Invasiveness and Stemness Properties by Inhibiting mTORC2/AKT.

Authors:  Carmen Mecca; Ileana Giambanco; Stefano Bruscoli; Oxana Bereshchenko; Bernard Fioretti; Carlo Riccardi; Rosario Donato; Cataldo Arcuri
Journal:  Front Cell Neurosci       Date:  2018-04-10       Impact factor: 5.505
  6 in total

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