| Literature DB >> 8236776 |
C R Dorn1, D H Francis, E J Angrick, J A Willgohs, R A Wilson, J E Collins, B H Jenke, S J Shawd.
Abstract
Isolates of Escherichia coli which produce Vero cytotoxin (VTEC) were obtained during 1983-1989 from calves raised in 5 north-central states of the USA. All of the calves experienced intestinal epithelial colonization by VTEC, diarrhea or both; twelve of the calves had bloody diarrhea. Twenty one isolates were serogroup O111 and the others were O103, O69, O45, 026, O5, or non-typable (4 isolates). All but one of the isolates hybridized with the CVD419 probe which identifies most VTEC strains. Thirty two isolates hybridized with the VT1 probe, 3 with both the VT1 and VT2 probes, and one with neither probe. The culture filtrate of the VT probe negative isolate was partially neutralized by SLT I monoclonal antibody. For the other isolates, the results of toxin neutralization by anti-SLT I and anti-SLT II monoclonal antibodies corresponded exactly with the VT1 and VT2 probe hybridization results. Three of the strains adhered in a localized manner to HEp-2 cells and Intestine 407 cells.Entities:
Mesh：
Substances：
Year: 1993 PMID： 8236776 PMCID： PMC7117345 DOI： 10.1016/0378-1135(93)90136-u
Source DB: PubMed Journal: Vet Microbiol ISSN： 0378-1135 Impact factor: 3.293