| Literature DB >> 8236389 |
A Tassanakajon1, V Boonsaeng, P Wilairat, S Panyim.
Abstract
The polymerase chain reaction (PCR) procedure using a primer set derived from a repetitive deoxyribonucleic acid (DNA) sequence specific to Plasmodium falciparum was used to detect parasite DNA in mosquitoes. In laboratory-infected mosquitoes, PCR could detect as few as 10 sporozoites in a dissected salivary gland and a single oocyst in a dissected midgut. The ability to detect P. falciparum DNA in wild-caught mosquitoes indicated an advantage of the PCR over enzyme-linked immunosorbent assay for the detection of Plasmodium sporozoites in mosquitoes with low-grade parasite infections.Entities:
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Year: 1993 PMID： 8236389 DOI： 10.1016/0035-9203(93)90124-9
Source DB: PubMed Journal: Trans R Soc Trop Med Hyg ISSN： 0035-9203 Impact factor: 2.184