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Literature DB >> 823529

Chloroplast DNA codes for transfer RNA.

Abstract

Transfer RNA's were isolated from Euglena gracilis. Chloroplast cistrons for tRNA were quantitated by hybridizing tRNA to ct DNA. Species of tRNA hybridizing to ct DNA were partially purified by hybridization-chromatography. The tRNA's hybridizing to ct DNA and nuclear DNA appear to be different. Total cellular tRNA was hybridized to ct DNA to an equivalent of approximately 25 cistrons. The total cellular tRNA was also separated into 2 fractions by chromatography on dihydroxyboryl substituted amino ethyl cellulose. Fraction I hybridized to both nuclear and ct DNA. Hybridizations to ct DNA indicated approximately 18 cistrons. Fraction II-tRNA hybridized only to ct DNA, saturating at a level of approximately 7 cistrons. The tRNA from isolated chloroplasts hybridized to both chloroplast and nuclear DNA. The level of hybridization to ct DNA indicated approximately 18 cistrons. Fraction II-type tRNA could not be detected in the isolated chloroplasts.

Entities: Chemical Species

Mesh：

Substances：

Year: 1976 PMID： 823529 PMCID： PMC343056 DOI： 10.1093/nar/3.8.2005

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

27 in total

1. Presence of N-formyl-methionyl-transfer RNA in bean chloroplasts.

Authors: G Burkard; B Eclancher; J H. Weil
Journal: FEBS Lett Date: 1969-08 Impact factor: 4.124

2. A deae-cellulose filter disk assay for aminoacyl-tRNA.

Authors: D V Santi; R T Anderson
Journal: Anal Biochem Date: 1974-03 Impact factor: 3.365

3. Hybridization of mitochondrial transfer RNA and mitochondrial DNA in petite mutants of yeast.

Authors: M Cohen; J Casey; M Rabinowitz; G S Getz
Journal: J Mol Biol Date: 1972-02-14 Impact factor: 5.469

4. Comparative studies of the tRNA's and the aminoacyl-tRNA synthetases from the cytoplasm and the chloroplasts of Phaseolus vulgaris.

Authors: G Burkard; P Guillemaut; J H Weil
Journal: Biochim Biophys Acta Date: 1970-11-12

5. In vitro iodination of DNA. Maximizing iodination while minimizing degradation; use of buoyant density shifts for DNA-DNA hybrid isolation.

Authors: J M Orosz; J G Wetmur
Journal: Biochemistry Date: 1974-12-31 Impact factor: 3.162

Review 6. The specificity of molecular hybridization reactions.

Authors: B J McCarthy; R B Church
Journal: Annu Rev Biochem Date: 1970 Impact factor: 23.643

7. Involvement of N-formylmethionine in initiation of protein synthesis in cell-free extracts of Euglena gracilis.

Authors: J H Schwartz; R Meyer; J M Eisenstadt; G Brawerman
Journal: J Mol Biol Date: 1967-05-14 Impact factor: 5.469

8. Covalent attachment of nucleic acids to agarose for affinity chromatography.

Authors: M S Poonian; A J Schlabach; A Weissbach
Journal: Biochemistry Date: 1971-02-02 Impact factor: 3.162

9. Studies on human tRNA. I. The rapid, large scale isolation and partial fractionation of placenta and liver tRNA.

Authors: B A Roe
Journal: Nucleic Acids Res Date: 1975-01 Impact factor: 16.971

10. A general method of gene isolation.

Authors: T Y Shih; M A Martin
Journal: Proc Natl Acad Sci U S A Date: 1973-06 Impact factor: 11.205

9 in total

1. Laboratory of Physiological Chemistry, State University Groningen, Netherlands.

Authors: H de Vries; J C de Jonge; H Bakker; H Meurs; A Kroon
Journal: Nucleic Acids Res Date: 1979 Impact factor: 16.971

2. Transfer RNA genes ofZea mays chloroplast DNA.

Authors: R F Selden; A Steinmetz; L McIntosh; L Bogorad; G Burkard; M Mubumbila; M Kuntz; E J Crouse; J H Weil
Journal: Plant Mol Biol Date: 1983-05 Impact factor: 4.076

3. Intraspecific variation in the structural organization and redundancy of chloroplast ribosomal DNA cistrons in Euglena gracilis.

Authors: E A Wurtz; D E Buetow
Journal: Curr Genet Date: 1981-07 Impact factor: 3.886

4. Analysis of Euglena gracilis chloroplast deoxyribonucleic acid with a restriction endonuclease, EcoRI.

Authors: J R Mielenz; J J Milner; C L Hershberger
Journal: J Bacteriol Date: 1977-05 Impact factor: 3.490

5. The nucleotide sequences of the genes coding for tRNAArgUCU, tRNAArgACG and tRNAAsnGUU on Spirodela oligorhiza chloroplast DNA.

Authors: R J Keus; N J Stam; T Zwiers; H T de Heij; G S Groot
Journal: Nucleic Acids Res Date: 1984-07-25 Impact factor: 16.971

9. Fractionation and identification of Euglena gracilis cytoplasmic and chloroplastic tRNAs and mapping of tRNA genes on chloroplast DNA.

Authors: M Kuntz; M Keller; E J Crouse; G Burkard; J H Weil
Journal: Curr Genet Date: 1982-10 Impact factor: 3.886